Literature DB >> 426762

Affinity chromatography of pig heart fumarase.

S Chaudhuri, E W Thomas.   

Abstract

2-(5'-Phenylpentyl)fumaric acid was shown to be a competitive inhibitor (Ki 0.5 mM) of pig heart fumarase. After nitration of the aromatic ring, reduction to the amine and diazotization, the acid was attached via azo linkages to a Sepharose 4B-tyramine matrix. The resulting adsorbent was used for the affinity chromatography of crude fumarase, purifications of approx. 20-fold being obtained by specific elution with 0.01 M-citrate.

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Year:  1979        PMID： 426762      PMCID： PMC1186345          DOI： 10.1042/bj1770115

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  8 in total

1.  The determination of enzyme inhibitor constants.

Authors:  M DIXON
Journal:  Biochem J       Date:  1953-08       Impact factor: 3.857

2.  The crystallization of fumarase.

Authors:  V MASSEY
Journal:  Biochem J       Date:  1952-07       Impact factor: 3.857

3.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

4.  Facile alkylation of methionine by benzyl bromide and demonstration of fumarase inactivation accompanied by alkylation of a methionine residue.

Authors:  G A Rogers; N Shaltiel; P D Boyer
Journal:  J Biol Chem       Date:  1976-09-25       Impact factor: 5.157

5.  Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.

Authors:  P Cuatrecasas
Journal:  J Biol Chem       Date:  1970-06       Impact factor: 5.157

6.  The substrate specificity of fumarase.

Authors:  J W Teipel; G M Hass; R L Hill
Journal:  J Biol Chem       Date:  1968-11-10       Impact factor: 5.157

7.  Multiple forms of fumarase of pig heart.

Authors:  Y C Lin; C F Scott; L H Cohen
Journal:  Arch Biochem Biophys       Date:  1971-06       Impact factor: 4.013

8.  A new purification procedure for fumarase based of affinity chromatography. Isolation and characterization of pig-liver fumarase.

Authors:  S Beeckmans; L Kanarek
Journal:  Eur J Biochem       Date:  1977-09
  8 in total
  1 in total

1.  Purification, characterization, and immunological properties of fumarase from Euglena gracilis var. bacillaris.

Authors:  H Shibata; W E Gardiner; S D Schwartzbach
Journal:  J Bacteriol       Date:  1985-11       Impact factor: 3.490
  1 in total

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