Literature DB >> 4264772

Isolation and characterization of myosin from cloned mouse fibroblasts.

R S Adelstein, M A Conti, G S Johnson, I Pastan, T D Pollard.   

Abstract

Myosin has been isolated from cloned mouse fibroblasts, line L-929. Fibroblast myosin: (i) binds to rabbit muscle actin and is dissociated from it by ATP, (ii) has an ATPase activity that is suppressed by Mg(2+) in 0.6 M KCl and is activated by rabbit muscle actin in the presence of Mg(2+) in 14 mM KCl, (iii) forms thin bipolar aggregates in 0.1 M KCl when viewed in the electron microscope, (iv) possesses a heavy chain with the same mobility as muscle myosin (molecular weight 200,000) in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In these respects, fibroblast myosin appears to be similar to muscle myosin in structure and function.

Entities:  

Mesh：

Substances：

Year:  1972        PMID： 4264772      PMCID： PMC389851          DOI： 10.1073/pnas.69.12.3693

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  15 in total

1.  The relationship between sulfhydryl groups and the activation of myosin adenosinetriphosphatase.

Authors:  W W KIELLEY; L B BRADLEY
Journal:  J Biol Chem       Date:  1956-02       Impact factor: 5.157

2.  Role of 3',5'-adenosine monophosphate in regulation of morphology and growth of transformed and normal fibroblasts.

Authors:  G S Johnson; I Pastan
Journal:  J Natl Cancer Inst       Date:  1972-05       Impact factor: 13.506

3.  Regulation of cell motility by cyclic AMP.

Authors:  G S Johnson; W D Morgan; I Pastan
Journal:  Nature       Date:  1972-01-07       Impact factor: 49.962

4.  Filament formation by purified Physarum myosin.

Authors:  V Nachmias
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

5.  Isolation and characterization of myosin and two myosin fragments from human blood platelets.

Authors:  R S Adelstein; T D Pollard; W M Kuehl
Journal:  Proc Natl Acad Sci U S A       Date:  1971-11       Impact factor: 11.205

6.  A phosphorylated light-chain component of myosin.

Authors:  W T Perrie; L B Smillie; S V Perry
Journal:  Biochem J       Date:  1972-07       Impact factor: 3.857

7.  Morphological transformation of Chinese hamster cells by dibutyryl adenosine cyclic 3':5'-monophosphate and testosterone.

Authors:  A W Hsie; T T Puck
Journal:  Proc Natl Acad Sci U S A       Date:  1971-02       Impact factor: 11.205

8.  Restoration of contact-inhibited growth to transformed cells by dibutyryl adenosine 3':5'-cyclic monophosphate.

Authors:  J R Sheppard
Journal:  Proc Natl Acad Sci U S A       Date:  1971-06       Impact factor: 11.205

9.  Formation of arrowhead complexes with heavy meromyosin in a variety of cell types.

Authors:  H Ishikawa; R Bischoff; H Holtzer
Journal:  J Cell Biol       Date:  1969-11       Impact factor: 10.539

10.  ATPase activity of myosin correlated with speed of muscle shortening.

Authors:  M Bárány
Journal:  J Gen Physiol       Date:  1967-07       Impact factor: 4.086
View more
  36 in total

1.  The actin content of fibroblasts.

Authors:  D Bray; C Thomas
Journal:  Biochem J       Date:  1975-05       Impact factor: 3.857

2.  Biochemistry of actomyosin-dependent cell motility (a review).

Authors:  E D Korn
Journal:  Proc Natl Acad Sci U S A       Date:  1978-02       Impact factor: 11.205

3.  Differences among myosins synthesized in non-myogenic cells, presumptive myoblasts, and myoblasts.

Authors:  J C Chi; S A Fellini; H Holtzer
Journal:  Proc Natl Acad Sci U S A       Date:  1975-12       Impact factor: 11.205

4.  Patterns of organization of actin and myosin in normal and transformed cultured cells.

Authors:  R Pollack; M Osborn; K Weber
Journal:  Proc Natl Acad Sci U S A       Date:  1975-03       Impact factor: 11.205

Review 5.  Leveraging the membrane - cytoskeleton interface with myosin-1.

Authors:  Russell E McConnell; Matthew J Tyska
Journal:  Trends Cell Biol       Date:  2010-05-12       Impact factor: 20.808

6.  Immunological evidence for the presence of smooth muscle-type contractile fibres in mouse macrophages.

Authors:  U Gröschel-Stewart; D Gröschel
Journal:  Experientia       Date:  1974-10-15

7.  Regulation of adenylate kinase and creatine kinase activities in myogenic cells.

Authors:  H Tarikas; D Schubert
Journal:  Proc Natl Acad Sci U S A       Date:  1974-06       Impact factor: 11.205

8.  Myosin is a component of the cell surface of cultured cells.

Authors:  M C Willingham; R E Ostlund; I Pastan
Journal:  Proc Natl Acad Sci U S A       Date:  1974-10       Impact factor: 11.205

9.  Muscle-like contractile proteins and tubulin in synaptosomes.

Authors:  A L Blitz; R E Fine
Journal:  Proc Natl Acad Sci U S A       Date:  1974-11       Impact factor: 11.205

10.  Phosphorylation of human platelet myosin.

Authors:  R S Adelstein; M A Conti; W Anderson
Journal:  Proc Natl Acad Sci U S A       Date:  1973-11       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.