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Literature DB >> 42441

Modulation of purified phospholipase A2 activity from human platelets by calcium and indomethacin.

Abstract

A membrane bound phospholipase A2 (phosphatide 2-acylhydrolase, EC 3.1.1.4) from human platelets has been purified 3500-fold, and partially characterized. Phospholipase A2 activity was assayed using [1(-14)C] oleate-labeled Escherichia coli or sonicated dispersions of synthetic phospholipids. The 2-acyl specificity of the phospholipase activity was confirmed using phosphatidylethanolamine labeled in the C-1 position as substrate. The purified enzyme was maximally active between pH 8.0 and 10.5, and had an absolute requirement for low concentrations of Ca2+. Indomethacin, but not aspirin, inhibited phospholipase A2 activity.

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Year: 1979 PMID： 42441 DOI： 10.1016/0005-2760(79)90117-6

Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002

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Cited

26 in total

1. Primary role of calcium ions in arachidonic acid release from rat platelet membranes. Comparison with human platelet membranes.

Authors: S Nakashima; A Suganuma; A Matsui; H Hattori; M Sato; A Takenaka; Y Nozawa
Journal: Biochem J Date: 1989-04-01 Impact factor: 3.857

2. Suppression of snake-venom cardiotoxin-induced cardiomyocyte degeneration by blockage of Ca2+ influx or inhibition of non-lysosomal proteinases.

Authors: W F Tzeng; Y H Chen
Journal: Biochem J Date: 1988-11-15 Impact factor: 3.857

8. Activation of human platelet phospholipase C by ionophore A23187 is totally dependent upon cyclo-oxygenase products and ADP.

Authors: S E Rittenhouse
Journal: Biochem J Date: 1984-08-15 Impact factor: 3.857

9. Phospholipid metabolism in stimulated human platelets. Changes in phosphatidylinositol, phosphatidic acid, and lysophospholipids.

Authors: M J Broekman; J W Ward; A J Marcus
Journal: J Clin Invest Date: 1980-08 Impact factor: 14.808

10. The effects of indomethacin and verapamil on the shape changes of vascular endothelial cells resulting from exposure to various inflammatory agents.

Authors: A M Northover
Journal: Agents Actions Date: 1988-07

Modulation of purified phospholipase A2 activity from human platelets by calcium and indomethacin.

1. Primary role of calcium ions in arachidonic acid release from rat platelet membranes. Comparison with human platelet membranes.

2. Suppression of snake-venom cardiotoxin-induced cardiomyocyte degeneration by blockage of Ca2+ influx or inhibition of non-lysosomal proteinases.

3. Distribution of pancreatic (group I) and synovial-type (group II) phospholipases A2 in human tissues.

4. Snake venom cardiotoxin can rapidly induce actin polymerization in intact platelets.

5. Allergen tachyphylaxis of guinea pigs in vivo; a prostaglandin E mediated phenomenon?

6. Incorporation of fatty acids into phospholipids in L cells stimulated by antibody.

7. Marked increase of human platelet phospholipase A2 activity in vitro and demonstration of an endogenous inhibitor.

8. Activation of human platelet phospholipase C by ionophore A23187 is totally dependent upon cyclo-oxygenase products and ADP.

9. Phospholipid metabolism in stimulated human platelets. Changes in phosphatidylinositol, phosphatidic acid, and lysophospholipids.

10. The effects of indomethacin and verapamil on the shape changes of vascular endothelial cells resulting from exposure to various inflammatory agents.