Literature DB >> 424314

Metabolic stability of 2' 5'oligo (A) and activity of 2' 5'oligo (A)-dependent endonuclease in extracts of interferon-treated and control HeLa cells.

M A Minks, S Benvin, P A Maroney, C Baglioni.   

Abstract

Extracts of interferon-treated HeLa cells adsorbed to poly(I) . poly(C)-agarose have been used to synthesize 2'5'oligo(A). This oligonucleotide has been characterized by enzymatic digestion with alkaline phosphatase, snake venom phosphodiesterase, T2 ribonuclease and chromatography on DEAE, and PEI-cellulose. The oligonucleotide inhibits protein synthesis in vitro and activates an endonuclease present in extracts of control and interferon-treated cells. The metabolic stability of 2'5'oligo(A) has been investigated in these cell extracts. The oligonucleotide undergoes rapid degradation, particularly in the absence of ATP and of an energy regenerating system. Furthermore, the 2'5'oligo(A)-activated endonuclease reverts to an inactive state under these conditions, but can be reactivated upon further addition of 2'5'oligo(A). A possible role for the degradation of 2'5'oligo(A) in the mechanism of interferon action is discussed.

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Year:  1979        PMID： 424314      PMCID： PMC327727          DOI： 10.1093/nar/6.2.767

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  22 in total

1.  Interferon-mediated protein kinase and low-molecular-weight inhibitor of protein synthesis.

Authors:  W K Roberts; A Hovanessian; R E Brown; M J Clemens; I M Kerr
Journal:  Nature       Date:  1976-12-02       Impact factor: 49.962

Review 2.  Antiviral activity of interferons.

Authors:  R M Friedman
Journal:  Bacteriol Rev       Date:  1977-09

3.  Nature of inhibitor of cell-free protein synthesis formed in response to interferon and double-stranded RNA.

Authors:  I M Kerr; R E Brown; A G Hovanessian
Journal:  Nature       Date:  1977-08-11       Impact factor: 49.962

4.  Specific phosphorylation in vitro of a protein associated with ribosomes of interferon-treated mouse L cells.

Authors:  A Zilberstein; P Federman; L Shulman; M Revel
Journal:  FEBS Lett       Date:  1976-09-15       Impact factor: 4.124

5.  Interferon action may be mediated by activation of a nuclease by pppA2'p5'A2'p5'A.

Authors:  C Baglioni; M A Minks; P A Maroney
Journal:  Nature       Date:  1978-06-22       Impact factor: 49.962

6.  Mechanism of interferon action. Properties of an interferon-mediated ribonucleolytic activity from mouse L929 cells.

Authors:  D A Eppstein; C E Samuel
Journal:  Virology       Date:  1978-08       Impact factor: 3.616

7.  pppA2'p5'A2'p5'A: an inhibitor of protein synthesis synthesized with an enzyme fraction from interferon-treated cells.

Authors:  I M Kerr; R E Brown
Journal:  Proc Natl Acad Sci U S A       Date:  1978-01       Impact factor: 11.205

8.  Inhibition of protein synthesis by Cl-.

Authors:  L A Weber; E D Hickey; P A Maroney; C Baglioni
Journal:  J Biol Chem       Date:  1977-06-10       Impact factor: 5.157

9.  Inhibition of cell-free protein synthesis by pppA2'p5'A2'p5'A: a novel oligonucleotide synthesized by interferon-treated L cell extracts.

Authors:  M J Clemens; B R Williams
Journal:  Cell       Date:  1978-03       Impact factor: 41.582

10.  Oligonucleotide inhibitor of protein synthesis made in extracts of interferon-treated chick embryo cells: comparison with the mouse low molecular weight inhibitor.

Authors:  L A Ball; C N White
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205
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  19 in total

1.  An interferon-induced phosphodiesterase degrading (2'-5') oligoisoadenylate and the C-C-A terminus of tRNA.

Authors:  A Schmidt; Y Chernajovsky; L Shulman; P Federman; H Berissi; M Revel
Journal:  Proc Natl Acad Sci U S A       Date:  1979-10       Impact factor: 11.205

2.  Mechanism for discrimination between viral and host mRNA in interferon-treated cells.

Authors:  T W Nilsen; C Baglioni
Journal:  Proc Natl Acad Sci U S A       Date:  1979-06       Impact factor: 11.205

3.  Activation of 2',5'-oligoadenylate synthetase and B-2 microglobulin in cancer patients treated with partially pure gamma interferon: dependence of biological effect on administration route.

Authors:  M G Rosenblum; A Riso; J U Gutterman
Journal:  Cancer Chemother Pharmacol       Date:  1986       Impact factor: 3.333

4.  Interferon, double-stranded RNA, and RNA degradation: activation of an endonuclease by (2'-5')An.

Authors:  E Slattery; N Ghosh; H Samanta; P Lengyel
Journal:  Proc Natl Acad Sci U S A       Date:  1979-10       Impact factor: 11.205

5.  Rapid chemical synthesis and circular dichroism properties of some 2'-5'-linked oligoriboadenylates.

Authors:  A F Markham; R A Porter; M J Gait; R C Sheppard; I M Kerr
Journal:  Nucleic Acids Res       Date:  1979-06-11       Impact factor: 16.971

6.  Influence of the xyloadenosine analogue of 2',5'-oligoriboadenylate on poly(A)-specific, 2',5'-oligoriboadenylate degrading 2',3'-exoribonuclease and further enzymes involved in poly(A)(+)mRNA metabolism.

Authors:  H C Schröder; G Gosselin; J L Imbach; W E Müller
Journal:  Mol Biol Rep       Date:  1984-12       Impact factor: 2.316

7.  Increased levels of (2'-5')oligo(A) polymerase activity in human lymphoblastoid cells treated with glucocorticoids.

Authors:  I Krishnan; C Baglioni
Journal:  Proc Natl Acad Sci U S A       Date:  1980-11       Impact factor: 11.205

Review 8.  The interferon renaissance: molecular aspects of induction and action.

Authors:  J Gordon; M A Minks
Journal:  Microbiol Rev       Date:  1981-06

9.  Heterogeneous nuclear RNA promotes synthesis of (2',5')oligoadenylate and is cleaved by the (2',5')oligoadenylate-activated endoribonuclease.

Authors:  T W Nilsen; P A Maroney; H D Robertson; C Baglioni
Journal:  Mol Cell Biol       Date:  1982-02       Impact factor: 4.272

10.  Elevated levels of (2'-5')oligoadenylic acid polymerase activity in growth-arrested human lymphoblastoid Namalva cells.

Authors:  I Krishnan; C Baglioni
Journal:  Mol Cell Biol       Date:  1981-10       Impact factor: 4.272
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