Literature DB >> 424294

Cytidine 5'-diphosphate reductase activity in phytohemagglutinin stimulated human lymphocytes.

G Tyrsted, V Gamulin.   

Abstract

The optimal conditions and the effect of deoxyribonucleoside triphosphates were determined for CDP reductase activity in PHA-stimulated lymphocytes. The enzymatic reaction showed an absolute requirement for ATP. In the absence of ATP, only dATP showed a minor stimulation of the reduction of CDP to dCDP. During transformation the CDP reductase activity reached a maximum at the same time as the four deoxyribonucleoside triphosphate pools, corresponding to mid S-phase at about 50 h after PHA addition. The DNA polymerase activity reached a maximum at 57 h.

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Year:  1979        PMID： 424294      PMCID： PMC327690          DOI： 10.1093/nar/6.1.305

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  26 in total

1.  The pool size of deoxyguanosine 5'-triphosphate and deoxycytidine 5'-triphosphate in phytohemagglutinin-stimulated and non-stimulated human lymphocytes.

Authors:  G Tyrsted
Journal:  Exp Cell Res       Date:  1975-03-15       Impact factor: 3.905

2.  A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors:  K BURTON
Journal:  Biochem J       Date:  1956-02       Impact factor: 3.857

3.  Induction of thymidine kinases in phytohaemagglutinin-stimulated human lymphocytes.

Authors:  B Munch-Petersen; G Tyrsted
Journal:  Biochim Biophys Acta       Date:  1977-10-04

4.  A convenient assay for ADP reductase activity using Dowex-1-borate columns.

Authors:  J G Cory; F A Russell; M M Mansell
Journal:  Anal Biochem       Date:  1973-10       Impact factor: 3.365

5.  Functional regulation of mammalian ribonucleotide reductase.

Authors:  H L Elford
Journal:  Adv Enzyme Regul       Date:  1972

6.  Ribonucleotide reductase and cell proliferation. I. Variations of ribonucleotide reductase activity with tumor growth rate in a series of rat hepatomas.

Authors:  H L Elford; M Freese; E Passamani; H P Morris
Journal:  J Biol Chem       Date:  1970-10-25       Impact factor: 5.157

7.  Cobamides and ribonucleotide reduction. V. A kinetic study of the ribonucleoside triphosphate reductase of Lactobacillus leichmannii.

Authors:  E Vitols; C Brownson; W Gardiner; R L Blakley
Journal:  J Biol Chem       Date:  1967-07-10       Impact factor: 5.157

8.  DNA polymerase activity in phytohemagglutinin-stimulated and non-stimulated human lymphocytes.

Authors:  G Tyrsted; B Munch-Petersen; L Cloos
Journal:  Exp Cell Res       Date:  1973-03-15       Impact factor: 3.905

9.  A rapid assay for CDP reductase activity in mammalian cell extracts.

Authors:  J R Steeper; C D Steuart
Journal:  Anal Biochem       Date:  1970-03       Impact factor: 3.365

10.  Regulation of fungal ribonucleotide reductase by unusual dinucleotides.

Authors:  W H Lewis; D R McNaughton; H B LéJohn; J A Wright
Journal:  Biochem Biophys Res Commun       Date:  1976-07-12       Impact factor: 3.575
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  2 in total

1.  Effect of 5-fluoro-2'-deoxyuridine and hydroxyurea on the phytohemagglutinin-induced increase of thymidine kinase, replicative DNA polymerase, deoxycytidylate deaminase and CDP reductase activities in human lymphocytes.

Authors:  G Tyrsted
Journal:  Mol Cell Biochem       Date:  1984-06       Impact factor: 3.396

2.  Deoxycytidylate deaminase activity in non-stimulated and phytohemagglutinin-stimulated human lymphocytes, and in leukemic cells.

Authors:  G Tyrsted; P C Chao; B Munch-Petersen
Journal:  Mol Cell Biochem       Date:  1987-07       Impact factor: 3.396
  2 in total

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