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Literature DB >> 42369

Protein synthesizing systems from spores and vegetative cells of Bacillus cereus.

Abstract

A system of polyphenylalanine synthesis was optimized for a comparison of the polymerizing activities of ribosomes from spores and vegetative cells of Bacillus cereus T. Ribosomes of both types react similarly, showing a magnesium optimum of about 6mM and spermidine optima of about 5mM and 4mM for vegative and spore ribosomes, respectively. These lead to optimum mono- to multivalent cation rations of 9 and 10 respectively at 100 mM ammonium ion. A comparison of the response of these ribosomes to suboptimal concentrations of magnesium and spermidine show that they differ qualitatively from each other, suggesting that they possess different structure, macromolecular or ionic components.

Entities: Chemical Disease Species

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Year: 1979 PMID： 42369 DOI： 10.1007/bf00425061

Source DB: PubMed Journal: Arch Microbiol ISSN： 0302-8933 Impact factor: 2.552

13 in total

4. Biochemical studies of bacterial sporulation and germination. XI. Protein-synthesizing systems from vegetative cells and spores of Bacillus megaterium.

Authors: M P Deutscher; P Chambon; A Konberg
Journal: J Biol Chem Date: 1968-10-10 Impact factor: 5.157

Protein synthesizing systems from spores and vegetative cells of Bacillus cereus.

1. Studies on spore germination: its independence from alanine racemase activity.

2. Cations and ribosome structure. 3. Effects on the 30S and 50S subunits of replacing bound Mg 2+ by inorganic cations.

3. Evidence for a defective protein synthesizing system in dormant spores of Bacillus cereus.

4. Biochemical studies of bacterial sporulation and germination. XI. Protein-synthesizing systems from vegetative cells and spores of Bacillus megaterium.

5. Ion-binding studies of ribonucleic acid and Escherichia coli ribosomes.

6. Ribosomes from spores of Bacillus cereus T.

7. The nature of ribosomes of spores of Bacillus cereus T. and Bacillus megaterium.

8. RNA CODEWORDS AND PROTEIN SYNTHESIS. THE EFFECT OF TRINUCLEOTIDES UPON THE BINDING OF SRNA TO RIBOSOMES.

9. Peptide synthesis by extracts from Bacillus subtilis spores.

10. Natural messenger ribonucleic acid-directed cell-free protein-synthesizing system of Bacillus subtilis.

1. Specificity of translation of spore polypeptides in bacillus in vitro systems.