Literature DB >> 4234788

Diagnosis of transmissible gastroenteritis in pigs by means of immunofluorescence.

M B Pensaert, E O Haelterman, T Burnstein.   

Abstract

THE FLUORESCENT ANTIBODY (FA) TEST FOR THE DIAGNOSIS OF FIELD OUTBREAKS OF TRANSMISSIBLE GASTROENTERITIS (TGE) IN BABY PIGS WAS COMPARED TO OTHER AVAILABLE MEANS INCLUDING: virus isolation by inoculation of test pigs, intestinal lesions especially villous atrophy, and clinical observations. Immunofluorescent tests were done on frozen sections of the small intestine and it was possible to make a specific diagnosis within two hours after collecting samples. The results obtained with the FA test compared favorably with virus isolation from infected tissues. It was considered a more advantageous procedure as long as infected pigs were in a relatively early phase of the disease. Because of the variability of the lesions as related to the stage of infection, pathologic diagnoses were less satisfactory. Field diagnoses made on the basis of clinical signs were least reliable.

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Mesh:

Year:  1968        PMID: 4234788      PMCID: PMC1319298     

Source DB:  PubMed          Journal:  Can J Comp Med        ISSN: 0008-4050


  10 in total

1.  RAPID DIAGNOSIS OF HOG CHOLERA: A TISSUE-IMPRESSION FLUORESCENT-ANTIBODY TECHNIQUE.

Authors:  J M AIKEN; K H HOOPES; E L STAIR; M B RHODES
Journal:  J Am Vet Med Assoc       Date:  1964-06-15       Impact factor: 1.936

2.  Fluorescent antibody staining of street and fixed rabies virus antigens.

Authors:  R A GOLDWASSER; R E KISSLING
Journal:  Proc Soc Exp Biol Med       Date:  1958-06

3.  Rapid diagnosis of human influenza infection from nasal smears by means of fluorescein-labeled antibody.

Authors:  C LIU
Journal:  Proc Soc Exp Biol Med       Date:  1956 Aug-Sep

4.  Studies on transmissible gastroenteritis of swine. II. Selected characteristics of a cytopathogenic virus common to five isolates from transmissible gastroenteritis.

Authors:  A W McClurkin; J O Norman
Journal:  Can J Comp Med Vet Sci       Date:  1966-07

5.  Use of fluorescein-labeled antibody form rapid diagnosis of transmissible gastroenteritis in experimentally infected pigs.

Authors:  S Konishi; R A Bankowski
Journal:  Am J Vet Res       Date:  1967-07       Impact factor: 1.156

6.  Detection of pseudorabies virus by immunofluorescence.

Authors:  W C Stewart; E A Carbrey; J I Kresse
Journal:  J Am Vet Med Assoc       Date:  1967-09-15       Impact factor: 1.936

7.  Growth of transmissible gastroenteritis virus in young pigs.

Authors:  B E Hooper; E O Haelterman
Journal:  Am J Vet Res       Date:  1966-01       Impact factor: 1.156

8.  Transmissible gastroenteritis of swine as a model for the study of enteric disease.

Authors:  E O Aelterman; B E Hooper
Journal:  Gastroenterology       Date:  1967-07       Impact factor: 22.682

9.  Studies of canine distemper infection by means of fluorescein-labeled antibody. II. The pathology and diagnosis of the naturally occurring disease in dogs and the antigenic nature of the inclusion body.

Authors:  D L COFFIN; C LIU
Journal:  Virology       Date:  1957-02       Impact factor: 3.616

10.  PREPARATION OF FLUORESCEIN ISOTHIOCYANATE-LABELED GAMMA-GLOBULIN BY DIALYSIS, GEL FILTRATION, AND IONEXCHANGE CHROMATOGRAPHY IN COMBINATION.

Authors:  R E DEDMON; A W HOLMES; F DEINHARDT
Journal:  J Bacteriol       Date:  1965-03       Impact factor: 3.490

  10 in total
  13 in total

1.  The use of immunofluorescence techniques for the laboratory diagnosis of transmissible gastroenteritis of swine.

Authors:  R F Solorzano; M Morin; L G Morehouse
Journal:  Can J Comp Med       Date:  1978-10

2.  Concurrent infection of pigs with enterotoxigenic Escherichia coli of different serogroups.

Authors:  D H Francis; R A Wilson
Journal:  J Clin Microbiol       Date:  1985-09       Impact factor: 5.948

3.  Transmissible gastroenteritis of swine: virus-intestinal cell interactions. I. Immunofluorescence, histopathology and virus production in the small intestine through the course of infection.

Authors:  M Pensaert; E O Haelterman; T Burnstein
Journal:  Arch Gesamte Virusforsch       Date:  1970

4.  Reovirus-like agent associated with fatal diarrhea in neonatal pigs.

Authors:  J G Lecce; M W King; R Mock
Journal:  Infect Immun       Date:  1976-09       Impact factor: 3.441

5.  An indirect fluorescent antibody test for antibodies to transmissible gastroenteritis of swine.

Authors:  D A Benfield; E O Haelterman; T Burnstein
Journal:  Can J Comp Med       Date:  1978-10

6.  A new coronavirus-like particle associated with diarrhea in swine.

Authors:  M B Pensaert; P de Bouck
Journal:  Arch Virol       Date:  1978       Impact factor: 2.574

7.  Transmissible gastroenteritis in feeder swine: clinical, immunofluorescence and histopathological obervations.

Authors:  M Morin; L G Morehouse; R F Solorzano; L D Olson
Journal:  Can J Comp Med       Date:  1973-07

8.  Propagation of the virus of porcine epidemic diarrhea in cell culture.

Authors:  M Hofmann; R Wyler
Journal:  J Clin Microbiol       Date:  1988-11       Impact factor: 5.948

9.  Detection of transmissible gastroenteritis virus by RT-PCR and differentiation from porcine respiratory coronavirus.

Authors:  D Paton; G Ibata; J Sands; A McGoldrick
Journal:  J Virol Methods       Date:  1997-07       Impact factor: 2.014

10.  An immunoelectron microscopic and immunofluorescent study on the antigenic relationship between the coronavirus-like agent, CV 777, and several coronaviruses.

Authors:  M B Pensaert; P Debouck; D J Reynolds
Journal:  Arch Virol       Date:  1981       Impact factor: 2.574

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