Comparison of several media for the isolation of Brucella.

Experiments were conducted to compare the efficacy of several media to isolate Br. abortus, Br. suis, and Br. melitensis from infected animal tissues. Greater differences among media were observed when infected tissues were the source of organisms than when in vitro propagated strains were used. All basal, enriched, and selective media used were adequate for isolation in most instances. However, enrichment of media with serum was necessary for maximum isolation of all strains of Brucella. Due to Brucella species and strain differences, employment of more than one medium was advantageous.