Literature DB >> 4218955

Proteolysis of rabbit immunoglobulin M by papain.

B M McIlroy, G T Stevenson.   

Abstract

1. Digestion of rabbit immunoglobulin M (IgM) by papain for 5h, in the absence of reducing agent, gives Fabmu and Fc(5)mu fragments in high yield. Shorter periods give fragment Fc(5)mu with one or more Fabmu fragments still attached. 2. Reducing agent in the absence of a denaturant cleaves rabbit IgM into half-subunits, each containing one mu chain and one light chain. Digestion by papain in the presence of such a reducing agent destroys the Fcmu domains.

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Year:  1974        PMID： 4218955      PMCID： PMC1168411          DOI： 10.1042/bj1430491

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  10 in total

1.  Action of papain on normal rabbit immunoglobulin M.

Authors:  H Ungar-Waron; J C Jaton; M Sela
Journal:  Biochim Biophys Acta       Date:  1967-08-15

2.  Complete amino acid sequence of the Mu heavy chain of a human IgM immunoglobulin.

Authors:  F W Putnam; G Florent; C Paul; T Shinoda; A Shimizu
Journal:  Science       Date:  1973-10-19       Impact factor: 47.728

3.  Precipitation of human serum proteins by polyethyleneglycol.

Authors:  B Chesebro; S E Svehag
Journal:  Clin Chim Acta       Date:  1968       Impact factor: 3.786

4.  Structure of human immunoglobuli M. II. Isolation of a high molecular weight Fc fragment of IgM composed of several Fc subunits.

Authors:  K Onoue; T Kishimoto; Y Yamamura
Journal:  J Immunol       Date:  1968-02       Impact factor: 5.422

5.  Agglutinating activities of pepsin fragments and subunits of IgM antibodies.

Authors:  T Kishimoto; K Onoue
Journal:  J Immunol       Date:  1971-02       Impact factor: 5.422

6.  Isolation of F(c)5mu and Fabmu fragments of human IgM.

Authors:  J Zikán; J C Bennett
Journal:  Eur J Immunol       Date:  1973-07       Impact factor: 5.532

7.  Immunoglobulin M: pentameric Fcmu fragments released by trypsin at higher temperatures.

Authors:  A G Plaut; T B Tomasi
Journal:  Proc Natl Acad Sci U S A       Date:  1970-02       Impact factor: 11.205

8.  PREPARATION AND PROPERTIES OF THE PEPTIDE CHAINS OF NORMAL HUMAN 19 S GAMMA-GLOBULIN (IGM).

Authors:  H CHAPLIN; S COHEN; E M PRESS
Journal:  Biochem J       Date:  1965-04       Impact factor: 3.857

9.  Immunoglobulin spots on the surface of rabbit lymphocytes.

Authors:  B Pernis; L Forni; L Amante
Journal:  J Exp Med       Date:  1970-11       Impact factor: 14.307

10.  Papain digestion fragments of human IgM globulins.

Authors:  C Mihaesco; M Seligmann
Journal:  J Exp Med       Date:  1968-03-01       Impact factor: 14.307
  10 in total
  1 in total

1.  Proteolysis of lymphocytic surface immunoglobulin.

Authors:  D W Hough; B M McIlroy; G T Stevenson
Journal:  Immunology       Date:  1977-03       Impact factor: 7.397
  1 in total

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