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Literature DB >> 4215080

The three-dimensional structure of the fab' fragment of a human myeloma immunoglobulin at 2.0-angstrom resolution.

R J Poljak, L M Amzel, B L Chen, R P Phizackerley, F Saul.

Abstract

The structural analysis of the Fab' fragment of human myeloma immunoglobulin IgGl(lambda) New has been extended to a nominal resolution of 2.0 A. Each of the structural subunits corresponding to the variable and to the constant homology regions of the light and heavy chains contains two irregular beta-sheets which are roughly parallel to each other and surround a tightly packed interior of hydrophobic side chains. About 50-60% of the amino-acid residues are included in beta-pleated sheets. Sequence alignments between the homology regions of Fab' New obtained by comparison of their three-dimensional structures are given. Some of the sequence variations observed in light and heavy chains and the role of the regions of hypervariable sequence in defining the size and shape of the active site of different immunoglobulin molecules are discussed on the basis of the three-dimensional model of Fab' New.

Entities: Disease Gene Species

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Year: 1974 PMID： 4215080 PMCID： PMC433789 DOI： 10.1073/pnas.71.9.3440

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

23 in total

1. Amino-acid sequence of the variable region of the heavy (alpha) chain of a mouse myeloma protein with anti-hapten activity.

Authors: S H Francis; R G Leslie; L Hood; H N Eisen
Journal: Proc Natl Acad Sci U S A Date: 1974-04 Impact factor: 11.205

2. Structure of Fab' New at 6 A resolution.

Authors: R J Poljak; L M Amzel; H P Avey; L N Becka
Journal: Nat New Biol Date: 1972-02-02

3. Three-dimensional structure of the Fab' fragment of a human immunoglobulin at 2,8-A resolution.

Authors: R J Poljak; L M Amzel; H P Avey; B L Chen; R P Phizackerley; F Saul
Journal: Proc Natl Acad Sci U S A Date: 1973-12 Impact factor: 11.205

4. Immunoglobulin G antibodies from an individual rabbit in which several heavy chain variants are paired with one light chain sequence.

Authors: B Friedenson; E Appella; Y Takeda; O A Roholt; D Pressman
Journal: J Biol Chem Date: 1973-10-25 Impact factor: 5.157

Review 8. Pyroglutamic acid. Non-metabolic formation, function in proteins and peptides, and characteristics of the enzymes effecting its removal.

Authors: G N Abraham; D N Podell
Journal: Mol Cell Biochem Date: 1981-08-11 Impact factor: 3.396

9. Partial amino acid sequences of kappa-chains of rat immunoglobulins: genetic and evolutionary implications.

Authors: A C Wang; H H Fudenberg; H Bazin
Journal: Biochem Genet Date: 1976-04 Impact factor: 1.890

10. Amino acid sequence of the Fv region of a human monoclonal IgM (protein WEA) with antibody activity against 3,4-pyruvylated galactose in Klebsiella polysaccharides K30 and K33.

Authors: F Goñi; B Frangione
Journal: Proc Natl Acad Sci U S A Date: 1983-08 Impact factor: 11.205

The three-dimensional structure of the fab' fragment of a human myeloma immunoglobulin at 2.0-angstrom resolution.

1. Amino-acid sequence of the variable region of the heavy (alpha) chain of a mouse myeloma protein with anti-hapten activity.

2. Structure of Fab' New at 6 A resolution.

3. Three-dimensional structure of the Fab' fragment of a human immunoglobulin at 2,8-A resolution.

4. Immunoglobulin G antibodies from an individual rabbit in which several heavy chain variants are paired with one light chain sequence.

5. Structure of a lambda-type Bence-Jones protein at 3.5-A resolution.

6. Electron microscopy of complexes between IgA (MOPC 315) and a bifunctional hapten.

7. Variability in the lambda light chain sequences of mouse antibody.

8. The covalent structure of an entire gammaG immunoglobulin molecule.

9. 2 -Microglobulin--a free immunoglobulin domain.

10. The three dimensional structure of a combining region-ligand complex of immunoglobulin NEW at 3.5-A resolution.

1. Diversity of light chain variable region sequences among rabbit antibodies elicited by the same antigens.

2. Possible palindromes in immunoglobulin heavy-chain genes: Their role in membrane attachment.

3. Isolation and characterization of the alpha platelet-derived growth factor receptor from rat olfactory epithelium.

4. Mechanisms of antibody diversity: multiple genes encode structurally related mouse kappa variable regions.

5. Alignment statistic for identifying related protein sequences.

Review 6. Biophysical highlights from 54 years of macromolecular crystallography.

7. Sequence variation among heavy chains from inulin-binding myeloma proteins.

Review 8. Pyroglutamic acid. Non-metabolic formation, function in proteins and peptides, and characteristics of the enzymes effecting its removal.

9. Partial amino acid sequences of kappa-chains of rat immunoglobulins: genetic and evolutionary implications.

10. Amino acid sequence of the Fv region of a human monoclonal IgM (protein WEA) with antibody activity against 3,4-pyruvylated galactose in Klebsiella polysaccharides K30 and K33.