Literature DB >> 4208551

Partial purification of detergent-soluble HL-A antigen and its cleavage by papain.

T A Springer, J L Strominger, D Mann.   

Abstract

HL-A antigen solubilized with the non-ionic detergent, Brij 99, has been purified to about 50% of homogeneity from a cultured human lymphoblast line. It consists of two nonidentical subunits of 44,000 and 12,000 molecular weight (MW). Upon papain proteolysis the 44,000 MW peptide is converted by at least two cleavages to a 34,000 MW peptide, but the 12,000 MW peptide appears to be unchanged. Concomitantly, the apparent molecular weight in gel filtration chromatography under nondenaturing conditions in the presence of Brij 99 is reduced from 460,000 to 45,000. HL-A molecules produced by direct papain proteolysis of membranes and by papain treatment of purified detergent-soluble HL-A are identical.

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Year:  1974        PMID： 4208551      PMCID： PMC388266          DOI： 10.1073/pnas.71.4.1539

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  35 in total

1.  Peptides obtained by tryptic hydrolysis of performic acid-oxidized ribonuclease.

Authors:  C H HIRS; S MOORE; W H STEIN
Journal:  J Biol Chem       Date:  1956-04       Impact factor: 5.157

2.  Three closely linked genetic systems relevant to transplantation.

Authors:  E J Yunis; D B Amos
Journal:  Proc Natl Acad Sci U S A       Date:  1971-12       Impact factor: 11.205

Review 3.  Biochemical properties of histocompatibility antigens.

Authors:  S G Nathenson
Journal:  Annu Rev Genet       Date:  1970       Impact factor: 16.830

4.  Lymphocyte activation and destruction in vitro in relation to MLC and HL-A.

Authors:  V P Eijsvoogel; R Du Bois; C J Melief; W P Zeylemaker; L Raat-Koning; L de Groot-Kooy
Journal:  Transplant Proc       Date:  1973-03       Impact factor: 1.066

5.  Characterization of soluble substances in plasma carrying HL-A alloantigenic activity and HL-A common antigenic activity.

Authors:  Y Miyakawa; N Tanigaki; V P Kreiter; G E Moore; D Pressman
Journal:  Transplantation       Date:  1973-03       Impact factor: 4.939

6.  Purification and characterization of HL-A antigens from human platelets, solubilized by the non-ionic detergent NP-40.

Authors:  A Dautigny; I Bernier; J Colombani; P Jollés
Journal:  Biochim Biophys Acta       Date:  1973-04-16

7.  The purification of detergent-solubilized HL-A antigens by affinity chromatography with the hemagglutinin from Lens culinaris.

Authors:  J R Dawson; J Silver; L B Sheppard; D B Amos
Journal:  J Immunol       Date:  1974-03       Impact factor: 5.422

8.  Isolation of H-2 alloantigens solubilized by the detergent NP-40.

Authors:  B D Schwartz; S G Nathenson
Journal:  J Immunol       Date:  1971-11       Impact factor: 5.422

9.  Studies on phytohemagglutinins. IV. Isolation and characterization of a hemagglutinin from the lentil, Lens, esculenta, Moench.

Authors:  M Tichá; G Entlicher; J V Kostír; J Kocourek
Journal:  Biochim Biophys Acta       Date:  1970-11-17

10.  The small subunit of HL-A antigens is beta 2-microglobulin.

Authors:  H M Grey; R T Kubo; S M Colon; M D Poulik; P Cresswell; T Springer; M Turner; J L Strominger
Journal:  J Exp Med       Date:  1973-12-01       Impact factor: 14.307
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  15 in total

1.  Inhibition of Epstein-Barr virus (EBV)-specific CD8+ cytotoxic T lymphocyte (CTL) activity by soluble HLA class I in vitro.

Authors:  B Gansuvd; M Hagihara; B Munkhbat; N Kanai; N Morita; N Munkhtuvshin; J Chargui; S Kato; T Hotta; K Tsuji
Journal:  Clin Exp Immunol       Date:  2000-01       Impact factor: 4.330

2.  Evidence for tryosine peptide homologies in the HLA antigens system.

Authors:  C Cunningham-Rundles; C Jersild; A Svejgaard; B Dupont; R A Good
Journal:  Proc Natl Acad Sci U S A       Date:  1975-12       Impact factor: 11.205

3.  Structure of HLA antigens: amino-acid and carbohydrate compositions and NH2-terminal sequences of four antigen preparations.

Authors:  C Terhorst; P Parham; D L Mann; J L Strominger
Journal:  Proc Natl Acad Sci U S A       Date:  1976-03       Impact factor: 11.205

4.  Evolutionary relationship between immunoglobulins and transplantation antigens.

Authors:  P A Peterson; L Rask; K Sege; L Klareskog; H Anundi; L Ostberg
Journal:  Proc Natl Acad Sci U S A       Date:  1975-04       Impact factor: 11.205

5.  Further structural studies of the heavy chain of HLA antigens and its similarity to immunoglobulins.

Authors:  C Terhorst; R Robb; C Jones; J L Strominger
Journal:  Proc Natl Acad Sci U S A       Date:  1977-09       Impact factor: 11.205

6.  Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids.

Authors:  R D Salter; D N Howell; P Cresswell
Journal:  Immunogenetics       Date:  1985       Impact factor: 2.846

7.  Detergent-soluble HLA antigens contain a hydrophilic region at the COOH-terminus and a penultimate hydrophobic region.

Authors:  T A Springer; J L Strominger
Journal:  Proc Natl Acad Sci U S A       Date:  1976-07       Impact factor: 11.205

8.  Common antigenic structures of HLA antigens. VII. Selective combination binding of beta2-microglobulin with HLA large component in cultured human cell lines.

Authors:  K Nakamuro; N Tanigaki; D Pressman
Journal:  Immunology       Date:  1977-02       Impact factor: 7.397

9.  Separation and comparison of human TL-like antigens and HLA(A, B, C) antigens expressed on cultured T cells.

Authors:  H Tokuyama; N Tanigaki
Journal:  Immunogenetics       Date:  1981       Impact factor: 2.846

10.  Immunological identity of the small subunit of HL-A antigens and beta2-microglobulin and its turnover on the cell membrane.

Authors:  P Cresswell; T Springer; J L Strominger; M J Turner; H M Grey; R T Kubo
Journal:  Proc Natl Acad Sci U S A       Date:  1974-05       Impact factor: 11.205
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