Literature DB >> 4207290

Studies on the fluorophore sempervirene and its complexes with DNA.

V W Burns.   

Abstract

Evidence that the fluorophore sempervirene binds to nucleic acids is presented. The complexes were studied by fluorescence intensity, spectra, decay lifetime, and polarization methods. Both fluorescent and nonfluorescent complexes are formed. The sempervirene is rigidly fixed to DNA. If ethidium bromide and sempervirene are bound to DNA, energy can be transferred from sempervirene to ethidium. Sempervirene is taken up by mammalian cells and appears in the cytoplasm. This unusual new probe should be useful in molecular and cellular investigations.

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Year:  1974        PMID： 4207290      PMCID： PMC1334494          DOI： 10.1016/s0006-3495(74)85906-0

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  9 in total

1.  Location and molecular characteristics of fluorescent complexes of ethidium bromide in the cell.

Authors:  V W Burns
Journal:  Exp Cell Res       Date:  1972-11       Impact factor: 3.905

2.  Fluorescence polarization characteristics of the complexes between ethidium bromide and rRNA, tRNA, and DNA.

Authors:  V W Burns
Journal:  Arch Biochem Biophys       Date:  1971-07       Impact factor: 4.013

3.  Fluorescence of proflavine--DNA complexes: heterogeneity of binding sites.

Authors:  J C Thomes; G Weill; M Daune
Journal:  Biopolymers       Date:  1969       Impact factor: 2.505

4.  Fluorescence decay time characteristics of the complex between ethidium bromide and nucleic acids.

Authors:  V W Burns
Journal:  Arch Biochem Biophys       Date:  1969-09       Impact factor: 4.013

5.  [Heterogeneity of binding sites of proflavin on DNA].

Authors:  J Chambron; R Bidet; G Weill
Journal:  Biopolymers       Date:  1971       Impact factor: 2.505

6.  Complex formation between ethidium bromide and nucleic acids.

Authors:  M J Waring
Journal:  J Mol Biol       Date:  1965-08       Impact factor: 5.469

7.  A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization.

Authors:  J B LePecq; C Paoletti
Journal:  J Mol Biol       Date:  1967-07-14       Impact factor: 5.469

8.  Fluorescence decay times: proteins, coenzymes, and other compounds in water.

Authors:  R F Chen; G G Vurek; N Alexander
Journal:  Science       Date:  1967-05-19       Impact factor: 47.728

9.  Interaction between nucleic acids and berberine sulfate.

Authors:  H YAMAGISHI
Journal:  J Cell Biol       Date:  1962-12       Impact factor: 10.539
  9 in total
  1 in total

1.  Sempervirine inhibits RNA polymerase I transcription independently from p53 in tumor cells.

Authors:  Cinzia Caggiano; Eugenia Guida; Federica Todaro; Pamela Bielli; Mattia Mori; Francesca Ghirga; Deborah Quaglio; Bruno Botta; Fabiola Moretti; Paola Grimaldi; Pellegrino Rossi; Emmanuele A Jannini; Marco Barchi; Susanna Dolci
Journal:  Cell Death Discov       Date:  2020-10-28
  1 in total

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