Literature DB >> 4198517

The purification and properties of extracellular glycosidases of the cellular slime mould Dictyostelium discoideum.

D Every, J M Ashworth.   

Abstract

The purification of beta-N-acetylhexosaminidase, alpha-glucosidase, alpha-mannosidase and beta-glucosidase from the spent growth medium of Dictyostelium discoideum strain Ax-2 myxamoebae is described. beta-N-Acetylhexosaminidase and alpha-glucosidase were obtained in high yield and as homogeneous preparations whereas the alpha-mannosidase preparation consisted of two electrophoretically distinct isoenzymes. The physical, chemical and kinetic properties of these enzymes are described.

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Year:  1973        PMID： 4198517      PMCID： PMC1177668          DOI： 10.1042/bj1330037

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  39 in total

1.  Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.

Authors:  A L Shapiro; E Viñuela; J V Maizel
Journal:  Biochem Biophys Res Commun       Date:  1967-09-07       Impact factor: 3.575

2.  Alpha-mannosidase and beta-mannosidase from the liver of Tubo cortunus: purfication, properties and application of carbohydrate research.

Authors:  T Muramatsu; F Egami
Journal:  J Biochem       Date:  1967-12       Impact factor: 3.387

3.  N-acetylhexosaminidases from a gastropod, Turbo cornutus.

Authors:  T Muramatsu
Journal:  J Biochem       Date:  1968-10       Impact factor: 3.387

4.  [Isolation, purification and some physicochemical characteristics of 2 beta-hexosidases of the digestive juice of Helix pomatia].

Authors:  R Got; A Marnay
Journal:  Eur J Biochem       Date:  1968-04-03

5.  Glycosidases of Phaseolus vulgaris. II. Isolation and general properties.

Authors:  K M Agrawal; O P Bahl
Journal:  J Biol Chem       Date:  1968-01-10       Impact factor: 5.157

6.  Acetylglucosaminidase, an early enzyme in the development of Dictyostelium discoideum.

Authors:  W F Loomis
Journal:  J Bacteriol       Date:  1969-03       Impact factor: 3.490

7.  Separation and properties of beta-galactosidase, beta-glucosidase, beta-glucuronidase and N-acetyl-beta-glucosaminidase from rat kidney.

Authors:  D Robinson; R G Price; N Dance
Journal:  Biochem J       Date:  1967-02       Impact factor: 3.857

8.  The gel-filtration behaviour of proteins related to their molecular weights over a wide range.

Authors:  P Andrews
Journal:  Biochem J       Date:  1965-09       Impact factor: 3.857

9.  Polysaccharides in vegetative and aggregation-competent amoebae of Dictyostelium discoideum. 2. Purification and characterization of amoeba-degraded bacterial polysaccharides.

Authors:  D Malchow; O Lüderitz; B Kickhöfen; O Westphal; G Gerisch
Journal:  Eur J Biochem       Date:  1969-01

10.  N-Acetyl-beta-glucosaminidases in human spleen.

Authors:  D Robinson; J L Stirling
Journal:  Biochem J       Date:  1968-04       Impact factor: 3.857
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  12 in total

1.  Glycosidases from the culture medium of Physarum polycephalum.

Authors:  D C Kilpatrick; J L Stirling
Journal:  Biochem J       Date:  1977-01-01       Impact factor: 3.857

2.  The structural gene for alpha-mannosidase-1 in Dictyostellium discoideum.

Authors:  S J Free; R T Schimke
Journal:  Genetics       Date:  1976-10       Impact factor: 4.562

3.  Rates of accumulation of glycosidase activities during growth and differentiation of Dictyostelium discoideum.

Authors:  D Every; J M Ashworth
Journal:  Biochem J       Date:  1975-05       Impact factor: 3.857

4.  Analysis of the patterns of expression of mRNAs for the alpha- and beta-subunits of the lysosomal enzyme beta-N-acetylhexosaminidase in mouse epididymis and testis.

Authors:  J L Stirling; T Beccari; J Hoade; F Pezzetti; M Calvitti; E Becchetti; A Orlacchio
Journal:  Histochem J       Date:  1991-10

5.  Mutations affecting N-acetylglucosaminidase in Dictyostelium discoideum.

Authors:  R L Dimond; M Brenner; W F Loomis
Journal:  Proc Natl Acad Sci U S A       Date:  1973-12       Impact factor: 11.205

6.  Identification and characterization of cells deficient in the mannose 6-phosphate receptor: evidence for an alternate pathway for lysosomal enzyme targeting.

Authors:  C A Gabel; D E Goldberg; S Kornfeld
Journal:  Proc Natl Acad Sci U S A       Date:  1983-02       Impact factor: 11.205

7.  Purification and properties of beta-N-acetylhexosaminidase from the mollusc Helicella ericetorum Müller.

Authors:  P Calvo; A Reglero; J A Cabezas
Journal:  Biochem J       Date:  1978-11-01       Impact factor: 3.857

8.  Properties and developmental regulation of an alpha-d-galactosidase from Dictyostelium discoideum.

Authors:  D C Kilpatrick; J L Stirling
Journal:  Biochem J       Date:  1976-08-15       Impact factor: 3.857

9.  Protein bodies and vacuoles as lysosomes : investigations into the role of mannose-6-phosphate in intracellular transport of glycosidases in pea cotyledons.

Authors:  P R Gaudreault; L Beevers
Journal:  Plant Physiol       Date:  1984-09       Impact factor: 8.340

10.  Rates of degradation and synthesis of glycosidases de novo during growth and differentiation of Dictyostelium discoideum.

Authors:  D Every; J M Ashworth
Journal:  Biochem J       Date:  1975-05       Impact factor: 3.857
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