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Literature DB >> 4196254

Deoxyribonucleic acid-membrane complexes in the Bacillus subtilis transformation system.

Abstract

The fate of transforming deoxyribonucleic acid (DNA) in Bacillus subtilis was studied by isolating the DNA-membrane complex on Renografin gradients. Soon after uptake, transforming DNA binds to the cell membrane and displays a greater resistance to shear than the recipient genome-membrane complex. Ten minutes after uptake, a portion of the donor DNA is released from the membrane. Most of the released donor radioactivity represents unintegrated, biologically inactive DNA. Recombinant or integrated DNA is enriched 1.5- to 1.7-fold in the membrane. This enrichment last at least 30 min after termination of DNA uptake, and probably much longer. The data suggest that transforming DNA may be integrated into the recipient genome on, or close to, the cell membrane.

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Year: 1973 PMID： 4196254 PMCID： PMC251831 DOI： 10.1128/jb.114.2.711-722.1973

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

24 in total

5. Fate of transforming DNA following uptake by competent Bacillus subtilis. IV. The endwise attachment and uptake of transforming DNA.

Authors: D Dubnau; C Cirigliano
Journal: J Mol Biol Date: 1972-02-28 Impact factor: 5.469

6. Association of the Bacillus subtilis chromosome with the cell membrane: resolution of free and bound deoxyribonucleic acid on renografin gradients.

Authors: R D Ivarie; J J Pène
Journal: J Bacteriol Date: 1970-11 Impact factor: 3.490

7. Macromolecular synthesis in Bacillus subtilis during development of the competent state.

Authors: D C Dooley; C T Hadden; E W Nester
Journal: J Bacteriol Date: 1971-11 Impact factor: 3.490

8. Evidence for the involvement of membranous bodies in the processes leading to genetic transformation in Bacillus subtilis.

Authors: D R Wolstenholme; C A Vermeulen; G Venema
Journal: J Bacteriol Date: 1966-10 Impact factor: 3.490

9. LAG PERIOD CHARACTERIZING THE ENTRY OF TRANSFORMING DEOXYRIBONUCLEIC ACID INTO BACILLUS SUBTILIS.

Authors: J S LEVINE; N STRAUSS
Journal: J Bacteriol Date: 1965-02 Impact factor: 3.490

10. CONFIGURATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID DURING ENTRY INTO BACILLUS SUBTILIS.

Authors: N STRAUSS
Journal: J Bacteriol Date: 1965-02 Impact factor: 3.490

7 in total

1. Characteristics of a complex formed by a nonintegrated fraction of transforming DNA and Bacillus subtilis recipient cell constituents.

Authors: D Pieniazek; M Piechowska; G Venema
Journal: Mol Gen Genet Date: 1977-11-18

2. Transformation of Bacillus subtilis: transforming ability of deoxyribonucleic acid in lysates of L-forms or protoplasts.

Authors: G E Bettinger; F E Young
Journal: J Bacteriol Date: 1975-06 Impact factor: 3.490

3. Transformation in bacillus subtilis: fate of transforming DNA in transformation deficient mutants.

Authors: J Buitenwerf; G Venema
Journal: Mol Gen Genet Date: 1977-03-07

4. Heterospecific transformation in Bacillus subtilis: protein composition of a membrane-DNA complex containing unstable heterologous donor-recipient complex.

Authors: H P te Riele; G Venema
Journal: Mol Gen Genet Date: 1984

5. In situ activity of enzymes on polyacrylamide gels of a deoxyribonucleic acid-membrane fraction extracted from pneumococci.

Authors: W Firshein
Journal: J Bacteriol Date: 1974-06 Impact factor: 3.490

6. Molecular fate of heterologous bacterial DNA in competent Bacillus subtilis: further characterization of unstable association between donor and recipient DNA and the involvement of the cellular membrane.