Literature DB >> 4191319

Nature of the ethylenediaminetetraacetic acid requirement for transformation of Bacillus subtilis with single-stranded deoxyribonucleic acid.

M J Tevethia, M Mandel.   

Abstract

The ethylenediaminetetraacetate (EDTA) requirement for transformation of Bacillus subtilis with single-stranded deoxyribonucleic acid (DNA) was examined. The results indicate that a chelating agent such as EDTA is a stringent requirement for transformation with single DNA strands only at nonsaturating DNA concentrations, and that EDTA, when required, must be present during several steps in the transformation process and appears to insure the survival of single-stranded DNA by rendering a nuclease in competent populations inactive.

Entities:  

Mesh：

Substances：

Year:  1970        PMID： 4191319      PMCID： PMC250400          DOI： 10.1128/jb.101.3.844-850.1970

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  11 in total

1.  A NONSPECIFIC INCREASE IN PERMEABILITY IN ESCHERICHIA COLI PRODUCED BY EDTA.

Authors:  L LEIVE
Journal:  Proc Natl Acad Sci U S A       Date:  1965-04       Impact factor: 11.205

2.  REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.

Authors:  C Anagnostopoulos; J Spizizen
Journal:  J Bacteriol       Date:  1961-05       Impact factor: 3.490

3.  Mechanism of the deoxyribonucleic acid helping effect during transformation.

Authors:  K C Chen; A W Ravin
Journal:  J Mol Biol       Date:  1968-05-14       Impact factor: 5.469

4.  Characterization of a naturally occurring, cross-linked fraction of DNA. 1. Nature of the cross-linkage.

Authors:  B M Alberts; P Doty
Journal:  J Mol Biol       Date:  1968-03-14       Impact factor: 5.469

5.  Efficient separation of single-stranded and double-stranded deoxyribonucleic acid in a dextran-polyethylene glycol two-phase system.

Authors:  B M Alberts
Journal:  Biochemistry       Date:  1967-08       Impact factor: 3.162

6.  Origin of the residual transforming activity of denatured Bacillus subtilis DNA.

Authors:  R Rownd; D M Green; R Sternglanz; P Doty
Journal:  J Mol Biol       Date:  1968-03-14       Impact factor: 5.469

7.  Further studies on transformation with single-stranded DNA of Hemophilus influenzae.

Authors:  E H Postel; S H Goodgal
Journal:  J Mol Biol       Date:  1967-09-14       Impact factor: 5.469

8.  Cellular site in Bacillus subtilis of a nuclease which preferentially degrades single-stranded nucleic acids.

Authors:  H C Birnboim
Journal:  J Bacteriol       Date:  1966-03       Impact factor: 3.490

9.  Residual transforming activity of denatured Haemophilus influenzae DNA.

Authors:  M R Chevallier; G Bernardi
Journal:  J Mol Biol       Date:  1968-03-14       Impact factor: 5.469

10.  Uptake of "single-stranded" DNA in Hemophilus influenzae and its ability to transform.

Authors:  E H Postel; S H Goodgal
Journal:  J Mol Biol       Date:  1966-04       Impact factor: 5.469
View more
  16 in total

Review 1.  Extracellular enzyme synthesis in the genus Bacillus.

Authors:  F G Priest
Journal:  Bacteriol Rev       Date:  1977-09

2.  Low-frequency rescue of a genetic marker in deoxyribonucleic acid from Bacillus bacteriophage phi 105 by superinfecting bacteriophage.

Authors:  L Rutberg; R W Armentrout
Journal:  J Virol       Date:  1970-12       Impact factor: 5.103

Review 3.  Bacteriophages of Bacillus subtilis.

Authors:  H E Hemphill; H R Whiteley
Journal:  Bacteriol Rev       Date:  1975-09

4.  Kinetic analysis of the products of donor deoxyribonucleate in transformed cells of Bacillus subtilis.

Authors:  R Davidoff-Abelson; D Dubnau
Journal:  J Bacteriol       Date:  1973-10       Impact factor: 3.490

5.  Interactions between exogenous deoxyribonucleic acid and membrane vesicles isolated from Bacillus subtilis 168.

Authors:  H Joenje; W N Konings; G Venema
Journal:  J Bacteriol       Date:  1974-09       Impact factor: 3.490

6.  Mature DNA from temperate bacillusphage phi105 requires primary recombination to be infectious in transfection.

Authors:  J I Flock; L Rutberg
Journal:  Mol Gen Genet       Date:  1974

7.  Pleiotropic effects of suppressor mutations in Bacillus subtilis.

Authors:  M J Tevethia; J N Baptist; M Mandel
Journal:  J Bacteriol       Date:  1974-09       Impact factor: 3.490

8.  Chromatographically fractionated complementary strands of Bacillus subtilis deoxyribonucleic acid: transformation of hybrids.

Authors:  R Rudner; V Remeza
Journal:  J Bacteriol       Date:  1973-02       Impact factor: 3.490

9.  Different nuclease activities in competent and noncompetent Bacillus subtilis.

Authors:  H Joenje; G Venema
Journal:  J Bacteriol       Date:  1975-04       Impact factor: 3.490

10.  Early intermediate state of transforming deoxyribonucleic acid during uptake by Bacillus subtilis.

Authors:  D A Morrison
Journal:  J Bacteriol       Date:  1971-10       Impact factor: 3.490
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.