Literature DB >> 4189973

Effect of the fluorescein to protein ratio on anti-Treponema pallidum conjugates.

S M Mothershed.   

Abstract

The effect of using different labeling techniques and various amounts of fluorescein-isothiocyanate was tested with several pools of antisera to Treponema pallidum. Optimal labeling occurred in all serum pools at a fluorescien to protein ratio of 15. The use of pure dye and the dialysis method of labeling is recommended as the method of choice because of its effectiveness in the reduction of nonspecific staining.

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Year:  1969        PMID： 4189973      PMCID： PMC378092          DOI： 10.1128/am.18.5.806-809.1969

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN： 0003-6919


  12 in total

1.  FLUORESCEIN-LABELED ANTIBODY TECHNIQUE FOR IDENTIFICATION OF LEPTOSPIRAL SEROTYPES--REFIXATION OF FORMALIN-FIXED ORGANISMS WITH OSMIC ACID VAPOR.

Authors:  W G DACRES
Journal:  Am J Vet Res       Date:  1963-11       Impact factor: 1.156

2.  A DIALYSIS TECHNIQUE FOR PREPARING FLUORESCENT ANTIBODY.

Authors:  H F CLARK; C C SHEPARD
Journal:  Virology       Date:  1963-08       Impact factor: 3.616

3.  Labeling procedures employing crystalline fluorescein isothiocyanate.

Authors:  C W GRIFFIN; T R CARSKI; G S WARNER
Journal:  J Bacteriol       Date:  1961-10       Impact factor: 3.490

4.  Technical factors affecting the preparation of fluorescent antibody reagents.

Authors:  L KAUFMAN; W B CHERRY
Journal:  J Immunol       Date:  1961-07       Impact factor: 5.422

5.  The quantitation of nonspecific staining as a guide for improvement of fluorescent antibody conjugates.

Authors:  B Pittman; G A Herbert; W B Cherry; G C Taylor
Journal:  J Immunol       Date:  1967-06       Impact factor: 5.422

6.  Factors affecting the degree of nonspecific staining given by fluorescein isothiocyanate labelled globulins.

Authors:  G A Hebert; B Pittman; W B Cherry
Journal:  J Immunol       Date:  1967-06       Impact factor: 5.422

7.  Preparation of fluorescein-labelled immune globulin for the identification of Treponema pallidum.

Authors:  S M Mothershed; J C Bullard
Journal:  Br J Vener Dis       Date:  1968-09

8.  Comparison of the VDRL slide, TPI, and FTA-ABS tests in experimental syphilis in rabbits.

Authors:  S M Mothershed; A R Yobs; J W Clark
Journal:  Br J Vener Dis       Date:  1967-12

9.  Comparison of concentration methods for fluorescent antibody reagents.

Authors:  V J Lewis; J B Brooks; W L Jones
Journal:  Am J Med Technol       Date:  1966 May-Jun

10.  Studies on fluorescent antibody staining. I. Non-specific fluorescence with fluorescein-coupled sheep anti-rabbit globulins.

Authors:  G GOLDSTEIN; I S SLIZYS; M W CHASE
Journal:  J Exp Med       Date:  1961-07-01       Impact factor: 14.307
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