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Literature DB >> 418384

On the biosynthesis of 5-methoxyuridine and uridine-5-oxyacetic acid in specific procaryotic transfer RNAs.

K Murao, H Ishikura, M Albani, H Kersten.

Abstract

The uridine-5-0-derivatives, 5-methoxyuridine (mo5U) and uridine-5-oxyacetic acid (cmo5U) occupy the first position of anticondons in certain tRNA species of B. subtilis and E. coli, respectively. Here we present experimental evidence showing that both modifications are derived from a common precursor, 5-hydroxyuridine. Incompletely modified tRNASer and tRNAVal from E. coli met- rel-. All five tRNAs accepted methyl groups from S-adenosylmethionine with B. subtilis extracts in vitro and mo5U was formed. In B. subtilis tRNAs the mo5U was proved to be at the specific site; in E. coli tRNAVal the mo5U was demonstrated to be present in the oligonucleotide that comprises the anticodon. In submethylated E. coli tRNAVal,5-hydroxyuridine was detected whereas considerable amounts of cmo5U were lacking.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1978 PMID： 418384 PMCID： PMC342075 DOI： 10.1093/nar/5.4.1273

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

20 in total

1. The nucleotide sequence of a serine tRNA from Escherichia coli.

Authors: H Ishikura; Y Yamada; S Nishimura
Journal: FEBS Lett Date: 1971-07-15 Impact factor: 4.124

2. Action of silkworm endonuclease on oligoribonucleotides terminating in 3'-phosphate.

Authors: J I Mukai; E I Soeta
Journal: Biochim Biophys Acta Date: 1967-03-29

3. Primary sequence of tRNA val from Escherichia coli B. I. Oligonucleotide sequences of digests of Escherichia coli tRNA val with RNase T and pancreatic RNase.

Authors: F Harada; F Kimura; S Nishimura
Journal: Biochemistry Date: 1971-08-17 Impact factor: 3.162

4. Purification and properties of Escherichia coli methyl-deficient phenylalanine tRNA.

Authors: N Biezunski; D Giveon; U Z Littauer
Journal: Biochim Biophys Acta Date: 1970-02-18

5. Uridin-5-oxy acetic acid: a new minor constituent from E. coli valine transfer RNA I.

Authors: K Murao; M Saneyoshi; F Harada; S Nishimura
Journal: Biochem Biophys Res Commun Date: 1970-02-20 Impact factor: 3.575

6. Separation of transfer ribonucleic acid by sepharose chromatography using reverse salt gradients.

Authors: W M Holmes; R E Hurd; B R Reid; R A Rimerman; G W Hatfield
Journal: Proc Natl Acad Sci U S A Date: 1975-03 Impact factor: 11.205

7. 5-Methoxyuridine, a new modified constituent in tRNAs of Bacillaceae.

Authors: M Albani; W Schmidt; H Kersten; K Geibel; I Lüderwald
Journal: FEBS Lett Date: 1976-11 Impact factor: 4.124

8. S-Adenosylmethionine and tetrahydrofolate-dependent methylation of tRNA in Bacillus subtilis. Incomplete methylations caused by trimethoprim, pactamycin, or chloramphenicol.

Authors: H H Arnold; W Schmidt; R Raettig; L Sandig; H Domdey; H Kersten
Journal: Arch Biochem Biophys Date: 1976-09 Impact factor: 4.013

9. Biosynthetic pathway of ribothymidine in B. subtilis and M. lysodeikticus involving different coenzymes for transfer RNA and ribosomal RNA.

Authors: W Schmidt; H H Arnold; H Kersten
Journal: Nucleic Acids Res Date: 1975-07 Impact factor: 16.971

On the biosynthesis of 5-methoxyuridine and uridine-5-oxyacetic acid in specific procaryotic transfer RNAs.

1. The nucleotide sequence of a serine tRNA from Escherichia coli.

2. Action of silkworm endonuclease on oligoribonucleotides terminating in 3'-phosphate.

3. Primary sequence of tRNA val from Escherichia coli B. I. Oligonucleotide sequences of digests of Escherichia coli tRNA val with RNase T and pancreatic RNase.

4. Purification and properties of Escherichia coli methyl-deficient phenylalanine tRNA.

5. Uridin-5-oxy acetic acid: a new minor constituent from E. coli valine transfer RNA I.

6. Separation of transfer ribonucleic acid by sepharose chromatography using reverse salt gradients.

7. 5-Methoxyuridine, a new modified constituent in tRNAs of Bacillaceae.

8. S-Adenosylmethionine and tetrahydrofolate-dependent methylation of tRNA in Bacillus subtilis. Incomplete methylations caused by trimethoprim, pactamycin, or chloramphenicol.

9. Biosynthetic pathway of ribothymidine in B. subtilis and M. lysodeikticus involving different coenzymes for transfer RNA and ribosomal RNA.

10. Isolation of 5-hydroxyuridine (iso-barbituridine) from yeast ribonucleic acids.

1. Chorismic acid, a key metabolite in modification of tRNA.

2. Hydrogen bond formation between the naturally modified nucleobase and phosphate backbone.

3. Identification of a novel tRNA wobble uridine modifying activity in the biosynthesis of 5-methoxyuridine.

4. Dual pathways of tRNA hydroxylation ensure efficient translation by expanding decoding capability.

5. S-Adenosyl-S-carboxymethyl-L-homocysteine: a novel cofactor found in the putative tRNA-modifying enzyme CmoA.