| Literature DB >> 417990 |
Abstract
Purification of tRNa nucleotidyltransferase from Lactobacillus acidophilus ATCC 4963 and Escherichia coli MRE 600 by preparative polyacrylamide gel electrophoresis is described. Both enzymes gave a single band on analytical polyacrylamide-gel electroesis and sodium dodecylsulfate gels. Chromatography of the high speed supernatant from Lactobacillus at low salt concentrations gave three enzyme fractions of molecular weights about 45 000, 90 000, and 120 000. At 1M NaCl only the first enzyme fraction was found. Kinetic data for both enzymes are given.Entities:
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Year: 1978 PMID： 417990 DOI： 10.1515/bchm.1978.359.1.473
Source DB: PubMed Journal: Hoppe Seylers Z Physiol Chem ISSN： 0018-4888