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Literature DB >> 417721

Demineralization in organic solvents by alkylammonium salts of ethylenediaminetetra-acetic acid.

Abstract

1. Insoluble salts of alklaine earth metals (e. g. calcium phosphate) dissolve in non-aqueous solvents containing EDTA salts of organic cations (e.g. tetraethylammonium). 2. Bone is efficiently demineralized in ethanolic trimethylammonium EDTA. Retention in the matrix of water-soluble materials (e.g. proteoglycan) is much better than in standard aqueous EDTA demineralization procedures.

Entities: Chemical

Mesh：

Substances：

Year: 1978 PMID： 417721 PMCID： PMC1183843 DOI： 10.1042/bj1690697

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

6 in total

1. A modified uronic acid carbazole reaction.

Authors: T BITTER; H M MUIR
Journal: Anal Biochem Date: 1962-10 Impact factor: 3.365

2. The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid.

Authors: J F WOESSNER
Journal: Arch Biochem Biophys Date: 1961-05 Impact factor: 4.013

3. Aliphatic ammonium salts in the assay of acidic polysaccharides from tissues.

Authors: J E SCOTT
Journal: Methods Biochem Anal Date: 1960

4. The isolation of soluble proteins, glycoproteins, and proteoglycans from bone.

Authors: G M Herring; B A Ashton
Journal: Prep Biochem Date: 1974

5. The estimation of acid glycosaminoglycan-Alcian blue complexes eluted from electrophoretic strips.

Authors: D J Newton; J E Scott; P Whiteman
Journal: Anal Biochem Date: 1974-11 Impact factor: 3.365

6. The recovery and characterization of acid glycosaminoglycans in normal human urine. Influence of a circadian rhythm.

Authors: J E Scott; D J Newton
Journal: Connect Tissue Res Date: 1975 Impact factor: 3.417

6 in total

15 in total

1. Load-induced proteoglycan orientation in bone tissue in vivo and in vitro.

Authors: T M Skerry; R Suswillo; A J el Haj; N N Ali; R A Dodds; L E Lanyon
Journal: Calcif Tissue Int Date: 1990-05 Impact factor: 4.333

2. Biochemical and immuno- and lectin-histochemical studies of solubility and retention of bone matrix proteins during EDTA demineralization.

Authors: M Takagi; M Maeno; Y Takahashi; K Otsuka
Journal: Histochem J Date: 1992-02

6. Ultrastructural and biochemical observations on proteoglycans and collagen in the mutable connective tissue of the feather star Antedon bifida (Echinodermata, Crinoidea).

Authors: R Erlinger; U Welsch; J E Scott
Journal: J Anat Date: 1993-08 Impact factor: 2.610

7. Effects of demineralization in an ethanolic solution of triethylammonium EDTA on solubility of bone matrix components and on ultrastructural preservation.

Authors: I R Dickson; S S Jande
Journal: Calcif Tissue Int Date: 1980 Impact factor: 4.333

8. Immunohistochemical localization of two otolith matrix proteins in the otolith and inner ear of the rainbow trout, Oncorhynchus mykiss: comparative aspects between the adult inner ear and embryonic otocysts.

Authors: Emi Murayama; Yasuaki Takagi; Hiromichi Nagasawa
Journal: Histochem Cell Biol Date: 2003-12-20 Impact factor: 4.304

9. Nature and distribution of chondroitin sulphate and dermatan sulphate proteoglycans in rabbit alveolar bone.

Authors: M Takagi; M Maeno; T Yamada; K Miyashita; K Otsuka
Journal: Histochem J Date: 1996-05

10. The structure of interfibrillar proteoglycan bridges (shape modules') in extracellular matrix of fibrous connective tissues and their stability in various chemical environments.

Authors: J E Scott; A M Thomlinson
Journal: J Anat Date: 1998-04 Impact factor: 2.610