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Literature DB >> 417139

Purification of human monocytes on microexudate-coated surfaces.

Abstract

Human blood monocytes, but not other Ficoll-Hypaque cells, adhere to plastic surfaces from which confluent BHK cells have been removed. EDTA (3mM) rapidly and completely removes monocytes, providing a simple technique for preparation of human monocytes in suspension. The method should be valuable for studies of monocyte function where monocyte monolayers are not suitable.

Entities: Chemical Species

Mesh：

Substances：
Ficoll
Edetic Acid

Year: 1978 PMID： 417139

Source DB: PubMed Journal: J Immunol ISSN： 0022-1767 Impact factor: 5.422

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Cited

50 in total

1. Monocytes/macrophages in diagnosis and immunopathogenesis.

Authors: S D Douglas
Journal: Clin Diagn Lab Immunol Date: 1999-05

2. Specific binding of an antigen-antibody complex to apoptotic human neutrophils.

Authors: Simon P Hart; Caroline Jackson; L Maximillian Kremmel; Mary S McNeill; Hubertus Jersmann; Karen M Alexander; James A Ross; Ian Dransfield
Journal: Am J Pathol Date: 2003-03 Impact factor: 4.307

3. Oxidative response of human neutrophils, monocytes, and alveolar macrophages induced by unopsonized surface-adherent Staphylococcus aureus.

Authors: M L Devalon; G R Elliott; W E Regelmann
Journal: Infect Immun Date: 1987-10 Impact factor: 3.441

4. Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1.

Authors: S H Smith; M H Brown; D Rowe; R E Callard; P C Beverley
Journal: Immunology Date: 1986-05 Impact factor: 7.397

5. Comparison of human monocytes isolated by elutriation and adherence suggests that heterogeneity may reflect a continuum of maturation/activation states.

Authors: I Dransfield; D Corcoran; L J Partridge; N Hogg; D R Burton
Journal: Immunology Date: 1988-03 Impact factor: 7.397

6. Comparative study of phagocytosis and intracellular bactericidal activity of human monocytes and polymorphonuclear cells. Application of fluorochrome and extracellular quenching technique.

Authors: W Pruzanski; S Saito
Journal: Inflammation Date: 1988-02 Impact factor: 4.092

Purification of human monocytes on microexudate-coated surfaces.

1. Monocytes/macrophages in diagnosis and immunopathogenesis.

2. Specific binding of an antigen-antibody complex to apoptotic human neutrophils.

3. Oxidative response of human neutrophils, monocytes, and alveolar macrophages induced by unopsonized surface-adherent Staphylococcus aureus.

4. Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1.

5. Comparison of human monocytes isolated by elutriation and adherence suggests that heterogeneity may reflect a continuum of maturation/activation states.

6. Comparative study of phagocytosis and intracellular bactericidal activity of human monocytes and polymorphonuclear cells. Application of fluorochrome and extracellular quenching technique.

7. Chemotactic factor-induced polarization, receptor redistribution, and locomotion of human blood monocytes.

8. Monoclonal antibodies specific for human monocytes, granulocytes and endothelium.

9. Distribution of 5'-nucleotidase in human lymphoid tissues.

10. Species restriction in cytostatic activity of human and murine monocytes and macrophages.