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Literature DB >> 4161881

Direct fluorescent labeling of microorganisms as a possible life-detection technique.

A Pital, S L Janowitz, C E Hudak, E E Lewis.

Abstract

Microorganisms and selected proteinaceous substances were directly tagged with fluorescein isothiocyanate. This approach suggested a possible application for detection of extraterrestrial life. A stable and apparently specific linkage was formed with protein, and nonprotein substances were readily destained. Soil and atmospheric debris did not exhibit any significant affinity for the dye.

Entities: Chemical

Mesh：

Substances：

Year: 1966 PMID： 4161881 PMCID： PMC546629 DOI： 10.1128/am.14.1.119-123.1966

Source DB: PubMed Journal: Appl Microbiol ISSN： 0003-6919

5 in total

1. ENHANCEMENT OF STAINING INTENSITY IN THE FLUORESCENT-ANTIBODY REACTION.

Authors: A PITAL; S L JANOWITZ
Journal: J Bacteriol Date: 1963-10 Impact factor: 3.490

2. Superiority of fluorescein isothiocyanate (Riggs) for fluorescent-antibody technic with a modification of its application.

Authors: J D MARSHALL; W C EVELAND; C W SMITH
Journal: Proc Soc Exp Biol Med Date: 1958 Aug-Sep

3. Interaction of enzymes with normal and tumour cells.

Authors: D H CORMACK; G C EASTY; E J AMBROSE
Journal: Nature Date: 1961-06-24 Impact factor: 49.962

4. Histological localization of fluorescent thyroidstimulating hormone in rat tissues.

Authors: E E MANCINI; O VILAR; J M DELLACHA; A GIMENO; A CASTRO
Journal: Nature Date: 1959-11-28 Impact factor: 49.962

5. Localization of antigen in tissue cells; cellular distribution of pneumococcal polysaccharides types II and III in the mouse.

Authors: M E KAPLAN; A H COONS; H W DEANE
Journal: J Exp Med Date: 1950-01-01 Impact factor: 14.307

5 in total

Direct fluorescent labeling of microorganisms as a possible life-detection technique.

1. ENHANCEMENT OF STAINING INTENSITY IN THE FLUORESCENT-ANTIBODY REACTION.

2. Superiority of fluorescein isothiocyanate (Riggs) for fluorescent-antibody technic with a modification of its application.

3. Interaction of enzymes with normal and tumour cells.

4. Histological localization of fluorescent thyroidstimulating hormone in rat tissues.

5. Localization of antigen in tissue cells; cellular distribution of pneumococcal polysaccharides types II and III in the mouse.

1. Persistence of Nosema locustae Spores in Soil as Determined by Fluorescence Microscopy.

2. Continuously variable amplitude contrast microscopy for the detection and study of microorganisms in soil.

3. Fluorescence and viability of Proteus mirabilis stained directly with fluorescein isothiocyanate.

4. Microorganisms in unamended soil as observed by various forms of microscopy and staining.

5. Accelerated immunofluorescence procedure for the detection of Salmonella in foods and animal by-products.