| Literature DB >> 4156360 |
Abstract
Membrane vesicles containing internal alcohol dehydrogenase and nicotinamide adenine dinucleotide (NAD) were prepared from Escherichia coli ML308-225. Upon the addition of ethanol, these vesicles respired and transported several amino acids. Transport rates driven by internally generated reduced NAD (NADH) were about 60 to 80% of that stimulated by d-lactate. This transport was inhibited by cyanide and anaerobiosis. Ferricyanide, a nonpermeable electron acceptor, inhibited transport stimulated by external NADH, but not that stimulated by internally generated NADH.Entities:
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Year: 1974 PMID: 4156360 PMCID: PMC245849 DOI: 10.1128/jb.120.2.861-865.1974
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490