Literature DB >> 415069

Continuous-flow automation of the Lactobacillus casei serum folate assay.

G B Tennant.   

Abstract

A method is described for the continuous-flow automation of the serum folate assay using Lactobacillus casei. The total incubation period is approximately four hours. The growth response of the organism to folate is estimated by measuring the rate of reduction of 2,3,5-triphenyl tetrazolium chloride (TTC). A simple continuous culture apparatus is used to grow the inoculum. Supplementation of the assay medium is necessary to obtain parallel results. A statistical assessment shows a favourable comparison with the whole-serum tube assay using a chloramphenicol resistant strain of L. casei. The method is less sensitive to inhibitory substances than the tube assay.

Entities:  

Mesh：

Substances：

Year:  1977        PMID： 415069      PMCID： PMC476703          DOI： 10.1136/jcp.30.12.1168

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  14 in total

1.  A microbiologic method for detecting folic acid deficiency in man.

Authors:  H BAKER; V HERBERT; O FRANK; I PASHER; S H HUTNER; L R WASSERMAN; H SOBOTKA
Journal:  Clin Chem       Date:  1959-08       Impact factor: 8.327

2.  STUDIES OF FOLATE DEFICIENCY IN MAN.

Authors:  V HERBERT
Journal:  Proc R Soc Med       Date:  1964-05

3.  Persistent excretion of pyrimethamine following oral administration.

Authors:  C C SMITH; J IHRIG
Journal:  Am J Trop Med Hyg       Date:  1959-01       Impact factor: 2.345

4.  The continuous culture of bacteria; a theoretical and experimental study.

Authors:  D HERBERT; R ELSWORTH; R C TELLING
Journal:  J Gen Microbiol       Date:  1956-07

5.  Automation of the assay of folate in serum and whole blood.

Authors:  L Millbank; R E Davis; M Rawlins; A H Waters
Journal:  J Clin Pathol       Date:  1970-02       Impact factor: 3.411

6.  Effect of incubation time on folate values.

Authors:  B A Slade; J W Harrison; W Shaw
Journal:  Am J Clin Pathol       Date:  1974-01       Impact factor: 2.493

7.  A comparison of seven strains of Lactobacillus casei var. rhamnosus in relation to their use in the microbiological assay of serum folate.

Authors:  G B Tennant; C H Newberry; J Davies; H Dziedzic
Journal:  J Appl Bacteriol       Date:  1974-09

8.  An assessment of work simplified procedures for the microbiological assay of serum vitamin B12 and serum folate.

Authors:  G B Tennant; J L Withey
Journal:  Med Lab Technol       Date:  1972-04

9.  Comparison of serum concentrations of methotrexate after various routes of administration.

Authors:  M Freeman-Narrod; B J Gerstley; P F Engstrom; R S Bornstein
Journal:  Cancer       Date:  1975-11       Impact factor: 6.860

10.  Studies on the folic acid activity of human serum.

Authors:  A H WATERS; D L MOLLIN
Journal:  J Clin Pathol       Date:  1961-07       Impact factor: 3.411
View more
  2 in total

1.  Estimation of serum folate by a radioassay and a continuous-flow method, compared with an established microbiological tume assay.

Authors:  J E O'Donnell; G B Tennant; B M Jones
Journal:  J Clin Pathol       Date:  1977-12       Impact factor: 3.411

2.  Anaemia in patients with myelomatosis.

Authors:  W C Ting; I Cavill; A Jacobs; S Kaaba; A May; S Smith; J A Whittaker
Journal:  Br J Cancer       Date:  1982-06       Impact factor: 7.640
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.