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Literature DB >> 415008

Purification of Pseudomonas aeruginosa exotoxin by affinity chromatography.

Abstract

Pseudomonas aeruginosa exotoxin A was purified by affinity chromatography from culture supernatants by elution of toxin from antitoxin immunoglobulin G-Sepharose 4B with 3 M NaSCN. The purity, toxicity, and enzymatic activity of exotoxin obtained were comparable to those of toxin purified by previously reported multiple-step procedures.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 1978 PMID： 415008 PMCID： PMC414049 DOI： 10.1128/iai.19.1.66-70.1978

Source DB: PubMed Journal: Infect Immun ISSN： 0019-9567 Impact factor: 3.441

21 in total

1. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors: B J DAVIS
Journal: Ann N Y Acad Sci Date: 1964-12-28 Impact factor: 5.691

2. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

3. Passive protection by antitoxin in experimental Pseudomonas aeruginosa burn infections.

Authors: O R Pavlovskis; M Pollack; L T Callahan; B H Iglewski
Journal: Infect Immun Date: 1977-12 Impact factor: 3.441

4. Use of microculture plates and the multiple automated sample harvester for in vitro microassay of bacterial toxins.

Authors: R C Knudsen; L T Callahan; A Ahmed; K W Sell
Journal: Appl Microbiol Date: 1974-08

5. Enzymatically active peptide from the adenosine diphosphate-ribosylating toxin of Pseudomonas aeruginosa.

Authors: D W Chung; R J Collier
Journal: Infect Immun Date: 1977-06 Impact factor: 3.441

6. Large-scale purification and characterization of the exotoxin of Pseudomonas aeruginosa.

Authors: S H Leppla
Journal: Infect Immun Date: 1976-10 Impact factor: 3.441

7. Neutralizing antibody to Pseudomonas aeruginosa exotoxin in human sera: evidence for in vivo toxin production during infection.

Authors: M Pollack; L T Callahan; N S Taylor
Journal: Infect Immun Date: 1976-10 Impact factor: 3.441

8. Serum antibody to Pseudomonas aeruginosa exotoxin measured by a passive hemagglutination assay.

Authors: M Pollack; N S Taylor
Journal: J Clin Microbiol Date: 1977-07 Impact factor: 5.948

9. Exotoxin production by clinical isolates of pseudomonas aeruginosa.

Authors: M Pollack; N S Taylor; L T Callahan
Journal: Infect Immun Date: 1977-03 Impact factor: 3.441

10. Incidence of exotoxin production by Pseudomonas species.

Authors: M J Bjorn; M L Vasil; J C Sadoff; B H Iglewski
Journal: Infect Immun Date: 1977-04 Impact factor: 3.441

14 in total

Review 1. Bacterial toxins: a table of lethal amounts.

Authors: D M Gill
Journal: Microbiol Rev Date: 1982-03

2. Enzyme-linked immunosorbent assay for Pseudomonas aeruginosa exotoxin A.

Authors: W W Schultz; T J Phipps; M Pollack
Journal: J Clin Microbiol Date: 1979-06 Impact factor: 5.948

3. Production of exoenzyme S during Pseudomonas aeruginosa infections of burned mice.

Authors: M J Bjorn; O R Pavlovskis; M R Thompson; B H Iglewski
Journal: Infect Immun Date: 1979-06 Impact factor: 3.441

4. Proteinases of Pseudomonas aeruginosa evoke mucin release by tracheal epithelium.

Authors: J D Klinger; B Tandler; C M Liedtke; T F Boat
Journal: J Clin Invest Date: 1984-11 Impact factor: 14.808

5. Protective activity of antibodies to exotoxin A and lipopolysaccharide at the onset of Pseudomonas aeruginosa septicemia in man.

Authors: M Pollack; L S Young
Journal: J Clin Invest Date: 1979-02 Impact factor: 14.808

6. In vitro inhibition of lymphocyte proliferation by Pseudomonas aeruginosa phenazine pigments.

Authors: R U Sorensen; J D Klinger; H A Cash; P A Chase; D G Dearborn
Journal: Infect Immun Date: 1983-07 Impact factor: 3.441

7. Toxin A-deficient mutants of Pseudomonas aeruginosa PA103: isolation and characterization.

Authors: D E Ohman; J C Sadoff; B H Iglewski
Journal: Infect Immun Date: 1980-06 Impact factor: 3.441

8. Functionally distinct monoclonal antibodies reactive with enzymatically active and binding domains of Pseudomonas aeruginosa toxin A.

Authors: J K Chia; M Pollack; D Avigan; S Steinbach
Journal: Infect Immun Date: 1986-06 Impact factor: 3.609

9. Targeted Delivery of Endosomal Escape Peptides to Enhance Immunotoxin Potency and Anti-cancer Efficacy.

Authors: Joseph Ryan Polli; Ping Chen; Brandon M Bordeau; Joseph P Balthasar
Journal: AAPS J Date: 2022-03-25 Impact factor: 3.603

10. Reaction of antibody in sera from cystic fibrosis patients with non-toxic forms of Pseudomonas aeruginosa exotoxin A.

Authors: K W Klinger; C W Shuster; J Klinger
Journal: Eur J Clin Microbiol Date: 1985-04 Impact factor: 5.103