Literature DB >> 414658

A counting method for determining the burst size of defective phages from Bacillus subtilis.

H Y Steensma, P J Sondermeijer.   

Abstract

The defective phages of Bacillus subtilis cannot be counted by plating as they do not form plaques. In addition, counting under the electron microscope with latex spheres as an internal standard is not possible. The reliability of a method using Escherichia coli phage T4 as a substitute for the latex spheres has been tested and the results compared with those of other methods. Using this method, we determined the burst sizes of the defective phages PBS X, PBS Y and PBS Z under various conditions.

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Year:  1977        PMID： 414658     DOI： 10.1007/bf02313757

Source DB:  PubMed          Journal:  Antonie Van Leeuwenhoek        ISSN： 0003-6072            Impact factor:   2.271


  15 in total

1.  Electron microscopic counting of virus particles by sedimentation on aluminized grids.

Authors:  K O SMITH; J L MELNICK
Journal:  J Immunol       Date:  1962-08       Impact factor: 5.422

2.  Electron micrographic counts of bacteriophage particles.

Authors:  S E LURIA; R C WILLIAMS; R C BACKUS
Journal:  J Bacteriol       Date:  1951-02       Impact factor: 3.490

3.  Molecular weights of coliphages and coliphage DNA. 3. Contour length and molecular weight of DNA from bacteriophages T4, T5 and T7, and from bovine papilloma virus.

Authors:  D Lang
Journal:  J Mol Biol       Date:  1970-12-28       Impact factor: 5.469

4.  Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.

Authors:  K R Yamamoto; B M Alberts; R Benzinger; L Lawhorne; G Treiber
Journal:  Virology       Date:  1970-03       Impact factor: 3.616

5.  Defective bacteriophage PBSH in Bacillus subtilis. I. Induction, purification, and physical properties of the bacteriophage and its deoxyribonucleic acid.

Authors:  M Haas; H Yoshikawa
Journal:  J Virol       Date:  1969-02       Impact factor: 5.103

6.  Calibration of molecular weight scales for DNA.

Authors:  S B Leighton; I Rubenstein
Journal:  J Mol Biol       Date:  1969-12-14       Impact factor: 5.469

7.  Defective bacteriophages.

Authors:  A J Garro; J Marmur
Journal:  J Cell Physiol       Date:  1970-12       Impact factor: 6.384

8.  Circular DNA from Shigella paradysenteriae.

Authors:  H S Ansz; J Zandberg; J H van de Pol; E F van Bruggen
Journal:  Eur J Biochem       Date:  1969-06

9.  Bacteriophages of Bacillus subtilis: comparison of different isolation techniques and possible use for classification of Bacillus subtilis strains.

Authors:  B K Rima; H Y Steensma
Journal:  Antonie Van Leeuwenhoek       Date:  1971       Impact factor: 2.271

10.  Properties of the defective phage of Bacillus subtilis.

Authors:  K Okamoto; J A Mudd; J Mangan; W M Huang; T V Subbaiah; J Marmur
Journal:  J Mol Biol       Date:  1968-06-28       Impact factor: 5.469
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  3 in total

1.  The occurrence and taxonomic value of PBS X-like defective phages in the genus Bacillus.

Authors:  H Y Steensma; L A Robertson; J D van Elsas
Journal:  Antonie Van Leeuwenhoek       Date:  1978       Impact factor: 2.271

2.  Characterization of a new Bacillus megaterium bacteriophage, MJ-1, from tropical soil.

Authors:  J D van Elsas; E G Penido
Journal:  Antonie Van Leeuwenhoek       Date:  1982       Impact factor: 2.271

3.  Cultivation-based assessment of lysogeny among soil bacteria.

Authors:  Kurt E Williamson; Jennifer B Schnitker; Mark Radosevich; David W Smith; K Eric Wommack
Journal:  Microb Ecol       Date:  2008-03-06       Impact factor: 4.552
  3 in total

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