Literature DB >> 4140507

Localization of Escherichia coli ribosomal proteins S4 and S14 by electron microscopy of antibody-labeled subunits.

J A Lake, M Pendergast, L Kahan, M Nomura.   

Abstract

Binding sites for antibodies specific for proteins S4 and S14 of the small subunit of E. coli ribosomes have been mapped on the surface of the subunit by electron microscopy. Antibody binding to reconstituted subunits was shown to depend specifically on the presence of E. coli S4 and S14. Anti-S14 IgG was found to bind to a limited region of the ribosome surface. In contrast anti-S4 IgG was found to bind to three separated regions of the ribosome surface, suggesting S4 has an elongated conformation in situ.

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Year:  1974        PMID： 4140507      PMCID： PMC433961          DOI： 10.1073/pnas.71.12.4688

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  18 in total

1.  Reconstitution of Escherichia coli 30 S ribosomal subunits from purified molecular components.

Authors:  W A Held; S Mizushima; M Nomura
Journal:  J Biol Chem       Date:  1973-08-25       Impact factor: 5.157

2.  The primary structure of ribosomal protein S4 from Escherichia coli.

Authors:  J Reinbolt; E Schiltz
Journal:  FEBS Lett       Date:  1973-11-01       Impact factor: 4.124

3.  The amino acid sequence of the N-terminal region of some 30S ribosomal proteins from Escherichia coli and Bacillus stearothermophilus: homologies in ribosomal proteins.

Authors:  M Yaguchi; C Roy; A T Matheson; L P Visentin
Journal:  Can J Biochem       Date:  1973-08

4.  Studies on the primary structure of 20 proteins from Escherichia coli ribosomes by means of an improved protein sequenator.

Authors:  B Wittmann-Liebold
Journal:  FEBS Lett       Date:  1973-11-01       Impact factor: 4.124

5.  Structure of liver ribosomes studied by negative staining.

Authors:  Y Nonomura; G Blobel; D Sabatini
Journal:  J Mol Biol       Date:  1971-09-14       Impact factor: 5.469

6.  Translation of the genetic message. VII. Role of initiation factors in formation of the chain initiation complex with Escherichia coli ribosomes.

Authors:  K Iwasaki; S Sabol; A J Wahba; S Ochoa
Journal:  Arch Biochem Biophys       Date:  1968-05       Impact factor: 4.013

7.  X-ray scattering study of ribosomes from Escherichia coli.

Authors:  W E Hill; J D Thompson; J W Anderegg
Journal:  J Mol Biol       Date:  1969-08-28       Impact factor: 5.469

8.  Functional correspondence between 30S ribosomal proteins of Escherichia coli and Bacillus stearothermophilus.

Authors:  K Higo; W Held; L Kahan; M Nomura
Journal:  Proc Natl Acad Sci U S A       Date:  1973-03       Impact factor: 11.205

9.  Sequence differences of Escherichia coli 30S ribosomal proteins as determined by immunochemical methods.

Authors:  G Stöffler; H G Wittmann
Journal:  Proc Natl Acad Sci U S A       Date:  1971-09       Impact factor: 11.205

10.  PREPARATION OF FLUORESCEIN ISOTHIOCYANATE-LABELED GAMMA-GLOBULIN BY DIALYSIS, GEL FILTRATION, AND IONEXCHANGE CHROMATOGRAPHY IN COMBINATION.

Authors:  R E DEDMON; A W HOLMES; F DEINHARDT
Journal:  J Bacteriol       Date:  1965-03       Impact factor: 3.490
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  22 in total

1.  Evidence for tertiary structural RNA-RNA interactions within the protein S4 binding site at the 5'-end of 16S ribosomal RNA of Escherichia coli.+.

Authors:  E Ungewickell; C Ehresmann; P Stiegler; R Garrett
Journal:  Nucleic Acids Res       Date:  1975-10       Impact factor: 16.971

2.  The characterisation of a fragment of ribosomal protein S4 that is protected against trypsin digestion by 16S ribosomal RNA of Escherichia coli.

Authors:  C Schulte; E Schiltz; R Garrent
Journal:  Nucleic Acids Res       Date:  1975-06       Impact factor: 16.971

3.  Site-directed hydroxyl radical probing of 30S ribosomal subunits by using Fe(II) tethered to an interruption in the 16S rRNA chain.

Authors:  R R Samaha; S Joseph; B O'Brien; T W O'Brien; H F Noller
Journal:  Proc Natl Acad Sci U S A       Date:  1999-01-19       Impact factor: 11.205

4.  Localization of the site of adenylylation of glutamine synthetase by electron microscopy of an enzyme-antibody complex.

Authors:  R J Frink; D Eisenberg; D G Glitz
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

5.  Localization of eukaryotic initiation factor 3 on native small ribosomal subunits.

Authors:  I Emanuilov; D D Sabatini; J A Lake; C Freienstein
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

6.  Localization of proteins S1, S2, S16 and S23 on the surface of small subunits of rat liver ribosomes by immune electron microscopy.

Authors:  G Lutsch; F Noll; H Theise; G Enzmann; H Bielka
Journal:  Mol Gen Genet       Date:  1979-10-03

7.  Ribosome structure: localization of 3' end of RNA in small subunit by immunoelectronmicroscopy.

Authors:  H M Olson; D G Glitz
Journal:  Proc Natl Acad Sci U S A       Date:  1979-08       Impact factor: 11.205

8.  Localization of the decoding region on the 30S Escherichia coli ribosomal subunit by affinity immunoelectron microscopy.

Authors:  M Keren-Zur; M Boublik; J Ofengand
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

9.  Ribosome structure: localization of N6,N6-dimethyladenosine by electron microscopy of a ribosome-antibody complex.

Authors:  S M Politz; D G Glitz
Journal:  Proc Natl Acad Sci U S A       Date:  1977-04       Impact factor: 11.205

10.  Location of protein S4 on the small ribosomal subunit of E. coli and B. stearothermophilus with protein- and hapten-specific antibodies.

Authors:  M Stöffler-Meilicke; B Epe; P Woolley; M Lotti; J Littlechild; G Stöffler
Journal:  Mol Gen Genet       Date:  1984
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