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Literature DB >> 4139705

Enzymatic synthesis of solid phase-bound DNA sequences corresponding to specific mammalian genes.

Abstract

With oligo(dT)-cellulose as primer, RNA-dependent DNA polymerase catalyzes the synthesis of cellulose-bound DNA that is complementary to mouse globin mRNA. The resulting cellulose-bound or solid phase complementary DNA hybridizes specifically with globin mRNA and permits the recovery of intact globin mRNA. This simple technique for the synthesis of solid phase-bound complementary DNA provides an additional and convenient method for the purification of specific genetic sequences.

Entities: Chemical Species

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Year: 1974 PMID： 4139705 PMCID： PMC434291 DOI： 10.1073/pnas.71.10.3892

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

11 in total

1. In vitro synthesis of DNA complementary to purified rabbit globin mRNA (RNA-dependent DNA polymerase-reticulocyte-hemoglobin-density gradient centrifugation-oligo(dT) primer).

Authors: J Ross; H Aviv; E Scolnick; P Leder
Journal: Proc Natl Acad Sci U S A Date: 1972-01 Impact factor: 11.205

2. In vitro synthesis of DNA components of human genes for globins.

Authors: A Bank; M Terada; S Metafora; L Dow; P A Marks
Journal: Nat New Biol Date: 1972-02-09

3. In vitro synthesis of DNA complementary to mRNA derived from a light chain-producing myeloma tumour.

Authors: H Aviv; S Packman; D Swan; J Ross; P Leder
Journal: Nat New Biol Date: 1973-02-07

4. Evidence for single copies of globin genes in the mouse genome.

Authors: P R Harrison; A Hell; G D Birnie; J Paul
Journal: Nature Date: 1972-09-22 Impact factor: 49.962

5. Reiteration frequency of haemoglobin genes in the duck.

Authors: J O Bishop; R Pemberton; C Baglioni
Journal: Nat New Biol Date: 1972-02-23

6. A comparison of globin genes in duck reticulocytes and liver cells.

Authors: S Packman; H Aviv; J Ross; P Leder
Journal: Biochem Biophys Res Commun Date: 1972-11-01 Impact factor: 3.575

7. Polynucleotide celluloses as solid state primers and templates for polymerases.

Authors: T M Jovin; A Kornberg
Journal: J Biol Chem Date: 1968-01-25 Impact factor: 5.157

8. A crude nuclease preparation suitable for use in DNA reassociation experiments.

Authors: W D Sutton
Journal: Biochim Biophys Acta Date: 1971-07-29

9. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

Authors: H Aviv; P Leder
Journal: Proc Natl Acad Sci U S A Date: 1972-06 Impact factor: 11.205

10. Primer requirement and template specificity of the DNA polymerase of RNA tumor viruses.

Authors: D Baltimore; D Smoler
Journal: Proc Natl Acad Sci U S A Date: 1971-07 Impact factor: 11.205

10 in total

1. Structure of a large segment of the genome of simian virus 40 that does not encode known proteins.

Authors: R Dhar; K N Subramanian; J Pan; S M Weissman
Journal: Proc Natl Acad Sci U S A Date: 1977-03 Impact factor: 11.205

2. Enrichment of special Novikoff hepatoma and regenerating liver mRNA by hybridization to cDNA-cellulose.

Authors: F W Hirsch; K N Nall; W H Spohn; H Busch
Journal: Proc Natl Acad Sci U S A Date: 1978-04 Impact factor: 11.205

3. A simple and efficient enzymatic method for covalent attachment of DNA to cellulose. Application for hybridization-restriction analysis and for in vitro synthesis of DNA probes.

Authors: T Goldkorn; D J Prockop
Journal: Nucleic Acids Res Date: 1986-11-25 Impact factor: 16.971

Enzymatic synthesis of solid phase-bound DNA sequences corresponding to specific mammalian genes.

1. In vitro synthesis of DNA complementary to purified rabbit globin mRNA (RNA-dependent DNA polymerase-reticulocyte-hemoglobin-density gradient centrifugation-oligo(dT) primer).

2. In vitro synthesis of DNA components of human genes for globins.

3. In vitro synthesis of DNA complementary to mRNA derived from a light chain-producing myeloma tumour.

4. Evidence for single copies of globin genes in the mouse genome.

5. Reiteration frequency of haemoglobin genes in the duck.

6. A comparison of globin genes in duck reticulocytes and liver cells.

7. Polynucleotide celluloses as solid state primers and templates for polymerases.

8. A crude nuclease preparation suitable for use in DNA reassociation experiments.

9. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

10. Primer requirement and template specificity of the DNA polymerase of RNA tumor viruses.

1. Structure of a large segment of the genome of simian virus 40 that does not encode known proteins.

2. Enrichment of special Novikoff hepatoma and regenerating liver mRNA by hybridization to cDNA-cellulose.

3. A simple and efficient enzymatic method for covalent attachment of DNA to cellulose. Application for hybridization-restriction analysis and for in vitro synthesis of DNA probes.

4. Use of molecular hybridization to purify and analyze albumin messenger RNA from rat liver.

5. Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.

6. The isolation of ecdysterone inducible genes by hybridization subtraction chromatography.

7. Purification of specific adenovirus 2 RNAs by preparative hybridization and selective thermal elution.

8. Reverse transcription of tRNA.

9. Regulation of albumin synthesis in rat liver.

10. Purification of a putative precursor of globin messenger RNA from mouse nucleated erythroid cells.