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Literature DB >> 4138854

Identification of chloroplast coupling factor by freeze-etching and negative-staining techniques.

Abstract

Identification of chloroplast coupling factor particles, by the freeze-etching and negative-staining techniques, was made utilizing chloroplast thylakoids isolated from spinach leaves. Complete removal of particles, comparable in diameter to purified coupling factor particles, from the outer surface of freeze-etched thylakoids was achieved by treatment with 0.8% silicotungstate. Reappearance of particles, comparable in diameter to purified coupling factor particles, on the outer surface of freeze-etched thylakoids was demonstrated by combining silicotungstate-treated thylakoids with purified chloroplast coupling factor. Negative-staining results were in agreement with the freeze-etch data. The results demonstrate that the chloroplast coupling factor particles are exposed on the outer surface.

Entities: Chemical Species

Mesh：

Substances：
Phosphotungstic Acid

Year: 1974 PMID： 4138854 PMCID： PMC2109332 DOI： 10.1083/jcb.63.1.24

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

13 in total

1. PARTIAL RESOLUTION OF THE ENZYMES CATALYZINE PHOTOPHOSPHORYLATION. I. STIMULATION OF PHOTOPHOSPHORYLATION BY A PREPARATION OF A LATENT, CA++- DEPENDENT ADENOSINE TRIPHOSPHATASE FROM CHLOROPLASTS.

Authors: V K VAMBUTAS; E RACKER
Journal: J Biol Chem Date: 1965-06 Impact factor: 5.157

2. Cell-membrane ultrastructure. Low-temperature electron microsopy and x-ray diffraction studies of lipoprotein components in lamellar systems.

Authors: H FERNANDEZ-MORAN
Journal: Circulation Date: 1962-11 Impact factor: 29.690

3. Freezing injury and uncoupling of phosphorylation from electron transport in chloroplasts.

Authors: U Heber
Journal: Plant Physiol Date: 1967-10 Impact factor: 8.340

4. Partial resolution of the enzymes catalyzing oxidative phosphorylation. XXI. Resolution of submitochondrial particles from bovine heart mitochondria with silicotungstate.

Authors: E Racker; L L Horstman; D Kling; J M Fessenden-Raden
Journal: J Biol Chem Date: 1969-12-25 Impact factor: 5.157

5. Hill reaction site in chloroplast membranes: non-participation of the quantasome particle in photoreduction.

Authors: S H Howell; E N Moudrianakis
Journal: J Mol Biol Date: 1967-07-28 Impact factor: 5.469

6. Partial resolution of the enzymes catalyzing photophosphorylation. 3. Activation of adenosine triphosphatase and 32P-labeled orthophosphate -adeno-sine triphosphate exchange in chloroplasts.

Authors: R E McCarty; E Racker
Journal: J Biol Chem Date: 1968-01-10 Impact factor: 5.157

7. Effect of a coupling factor and its antiserum on photophosphorylation and hydrogen ion transport.

Authors: R E McCarty; E Racker
Journal: Brookhaven Symp Biol Date: 1966

8. Partial resolution of the enzymes catalyzing photophosphorylation. 8. Properties of silicotungstate-treated subchloroplast particles.

Authors: S Lien; E Racker
Journal: J Biol Chem Date: 1971-07-10 Impact factor: 5.157

9. Theoretical and experimental effects of cooling and warming velocity on the survival of frozen and thawed cells.

Authors: P Mazur
Journal: Cryobiology Date: 1966 Jan-Feb Impact factor: 2.487

10. A method for increasing contrast of mitochondrial inner membrane spheres in thin sections of epon-araldite embedded tissue.

Authors: J N Telford; E Racker
Journal: J Cell Biol Date: 1973-05 Impact factor: 10.539

8 in total

5. Effect of Light and Chilling Temperatures on Chilling-sensitive and Chilling-resistant Plants. Pretreatment of Cucumber and Spinach Thylakoids in Vivo and in Vitro.

Authors: M P Garber
Journal: Plant Physiol Date: 1977-05 Impact factor: 8.340

Identification of chloroplast coupling factor by freeze-etching and negative-staining techniques.

1. PARTIAL RESOLUTION OF THE ENZYMES CATALYZINE PHOTOPHOSPHORYLATION. I. STIMULATION OF PHOTOPHOSPHORYLATION BY A PREPARATION OF A LATENT, CA++- DEPENDENT ADENOSINE TRIPHOSPHATASE FROM CHLOROPLASTS.

2. Cell-membrane ultrastructure. Low-temperature electron microsopy and x-ray diffraction studies of lipoprotein components in lamellar systems.

3. Freezing injury and uncoupling of phosphorylation from electron transport in chloroplasts.

4. Partial resolution of the enzymes catalyzing oxidative phosphorylation. XXI. Resolution of submitochondrial particles from bovine heart mitochondria with silicotungstate.

5. Hill reaction site in chloroplast membranes: non-participation of the quantasome particle in photoreduction.

6. Partial resolution of the enzymes catalyzing photophosphorylation. 3. Activation of adenosine triphosphatase and 32P-labeled orthophosphate -adeno-sine triphosphate exchange in chloroplasts.

7. Effect of a coupling factor and its antiserum on photophosphorylation and hydrogen ion transport.

8. Partial resolution of the enzymes catalyzing photophosphorylation. 8. Properties of silicotungstate-treated subchloroplast particles.

9. Theoretical and experimental effects of cooling and warming velocity on the survival of frozen and thawed cells.

10. A method for increasing contrast of mitochondrial inner membrane spheres in thin sections of epon-araldite embedded tissue.

1. Three proton pumps, morphology and movements.

2. Multilamellar surface layer of the cell wall of Albugo candida and Phycomyces blakesleeanus.

3. Visualization of mitochondrial coupling factor F1(ATPase) by freeze-drying.

4. Alterations in Chloroplast Thylakoids during Cold Acclimation.

5. Effect of Light and Chilling Temperatures on Chilling-sensitive and Chilling-resistant Plants. Pretreatment of Cucumber and Spinach Thylakoids in Vivo and in Vitro.

6. Alterations in Chloroplast Thylakoids during an in Vitro Freeze-Thaw Cycle.

Review 7. Recent developments on structural and functional aspects of the F1 sector of H+-linked ATPases.

8. Reversible particle movements associated with unstacking and restacking of chloroplast membranes in vitro.