Literature DB >> 4135851

Improved techniques for staining and enumerating focus formation in viral infectivity assays.

D J Self, K F Pettiss, D D Bigner.   

Abstract

Improved techniques consisting of glutaraldehyde fixation, Giemsa staining, and colony counter enumeration of foci in viral infectivity assays were compared to microscope scanning and lantern slide projection methods. These techniques broadened the scope of application to potentially hazardous viruses and increased the efficiency of assaying.

Entities:  

Mesh:

Substances:

Year:  1974        PMID: 4135851      PMCID: PMC186615          DOI: 10.1128/am.28.1.156-157.1974

Source DB:  PubMed          Journal:  Appl Microbiol        ISSN: 0003-6919


  3 in total

1.  Characteristics of an assay for Rous sarcoma virus and Rous sarcoma cells in tissue culture.

Authors:  H M TEMIN; H RUBIN
Journal:  Virology       Date:  1958-12       Impact factor: 3.616

2.  Discrete foci of altered chicken embryo cells associated with Rous sarcoma virus in tissue culture.

Authors:  V GROUPE; R A MANAKER
Journal:  Virology       Date:  1956-12       Impact factor: 3.616

3.  An improved method for enumeration of X-C cell assay for murine leukemia virus.

Authors:  G J Spahn; R M Nims; R L Peters; K Kenyon
Journal:  Appl Microbiol       Date:  1973-01
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.