Literature DB >> 4113261

New in-vitro test for IgE-mediated hypersensitivity in man.

M W Greaves, S Yamamoto, V M Fairley.   

Abstract

A new simple and sensitive in-vitro method for the diagnosis of type 1 (IgE-mediated) hypersensitivity in man is described. Sliced human skin is passively sensitized by reaginic serum from allergic patients and the presence of antigen-specific IgE on the sensitized slices is detected by assay of antigen-evoked histamine release. Serum from 12 out of 14 patients with clinical respiratory allergy and positive skin tests gave significant antigen-specific histamine release. This method, which is essentially an in-vitro model of the Prausnitz-Küstner reaction, should prove of value in the diagnosis of human reaginic hypersensitivity in man.

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Year:  1972        PMID： 4113261      PMCID： PMC1788399          DOI： 10.1136/bmj.2.5814.623

Source DB:  PubMed          Journal:  Br Med J        ISSN： 0007-1447


  5 in total

1.  A study of provocation tests on patients with bronchial asthma. I. The reliability of provocation tests performed under different conditions.

Authors:  H COLLDAHL
Journal:  Acta Allergol       Date:  1952

2.  Release of histamine and slow reacting substance with mast cell changes after challenge of human lung sensitized passively with reagin in vitro.

Authors:  W E Parish
Journal:  Nature       Date:  1967-08-12       Impact factor: 49.962

3.  Diagnosis of allergy by an in-vitro test for allergen antibodies.

Authors:  L Wide; H Bennich; S G Johansson
Journal:  Lancet       Date:  1967-11-25       Impact factor: 79.321

4.  Use of antibody-coupled cellulose as immunosorbent in the estimation of human immunoglobulin E ( E).

Authors:  P McLaughlan; D R Stanworth; J F Kennedy; H Cho Tun
Journal:  Nat New Biol       Date:  1971-08-25

5.  Detection of allergy to penicillin and other antigens by in-vitro passive sensitization and histamine release from human and monkey lung.

Authors:  E S Assem; H O Schild
Journal:  Br Med J       Date:  1968-08-03
  5 in total
  2 in total

1.  [Immunoglobulin E. Biochemical, immunological properties and clinical significance (author's transl)].

Authors:  R Lamerz; A Fateh-Moghadam
Journal:  Klin Wochenschr       Date:  1974-01-01

2.  IgE-mediated hypersensitivity in human skin studied using a new in vitro method.

Authors:  M W Greaves; S Yamamoto; V M Fairley
Journal:  Immunology       Date:  1972-08       Impact factor: 7.397
  2 in total

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