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Literature DB >> 4108524

Biochemical differentiation in reaggregating brain cell culture.

Abstract

Dissociated cells from embryonic mouse brain reassociate in rotation culture to form aggregates. During cell culture the specific activities of choline acetyl-transferase (EC 2.3.1.6), acetylcholinesterase (EC 3.1.1.7), and glutamate decarboxylase (EC 4.1.1.15) in the aggregates increase up to twenty-fold, a phenomenon that approximates some of the biochemical events in the development of the mouse brain.

Entities: Gene Species

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Year: 1971 PMID： 4108524 PMCID： PMC389308 DOI： 10.1073/pnas.68.8.1858

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

17 in total

1. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

2. The determination of cholinesterase activity in whole brains of developing rats.

Authors: C J METZLER; D G HUMM
Journal: Science Date: 1951-04-06 Impact factor: 47.728

3. The formation of synaptic junctions in developing rat brain: a quantitative electron microscopic study.

Authors: G K Aghajanian; F E Bloom
Journal: Brain Res Date: 1967-12 Impact factor: 3.252

4. Regulation of acetylcholinesterase in neuroblastoma cells.

Authors: A Blume; F Gilbert; S Wilson; J Farber; R Rosenberg; M Nirenberg
Journal: Proc Natl Acad Sci U S A Date: 1970-10 Impact factor: 11.205

5. Histogenesis of fetal mouse isocortex and hippocampus in reaggregating cell cultures.

Authors: G R DeLong
Journal: Dev Biol Date: 1970-08 Impact factor: 3.582

6. Regulation of axon formation by clonal lines of a neural tumor.

Authors: N W Seeds; A G Gilman; T Amano; M W Nirenberg
Journal: Proc Natl Acad Sci U S A Date: 1970-05 Impact factor: 11.205

7. Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.

Authors: F T Kao; T T Puck
Journal: Proc Natl Acad Sci U S A Date: 1968-08 Impact factor: 11.205

8. A simplified radiochemical assay for choline acetyltransferase.

Authors: B K Schrier; L Shuster
Journal: J Neurochem Date: 1967-10 Impact factor: 5.372

9. Differentiated rat glial cell strain in tissue culture.

Authors: P Benda; J Lightbody; G Sato; L Levine; W Sweet
Journal: Science Date: 1968-07-26 Impact factor: 47.728

10. L-glutamic acid decarboxylase: a new type in glial cells and human brain gliomas.

Authors: B Haber; K Kuriyama; E Roberts
Journal: Science Date: 1970-05-01 Impact factor: 47.728

19 in total

1. Dissociated cell cultures from fetal mouse hypothalamus. Patterns of organization and ultrastructural features.

Authors: P Benda; F De Vitry; R Picart; A Tixier-Vidal
Journal: Exp Brain Res Date: 1975-07-11 Impact factor: 1.972

2. The advantage of the aggregate culture of isolated ovarian cell types over the monolayer culture.

Authors: E L Gregoraszczuk
Journal: Cytotechnology Date: 1990-09 Impact factor: 2.058

3. Ultrastructural investigation of fetal rat brain hemisphere tissue in nonadherent stationary organ culture.

Authors: S K Steinsvåg
Journal: Experientia Date: 1986-07-15

4. Light and electron microscopic demonstration of cholinesterases of the cultured sympathetic ganglia of the chick embryo.

Authors: H Hervonen; L Rechardt
Journal: Histochemistry Date: 1974-04-22