Literature DB >> 41020

A combined alkali extraction--ethidium bromide technique for the measurement of DNA in small pieces of tissue.

K M Halprin, J R Taylor, V Levine, K Adachi.   

Abstract

Alkaline solutions (0.1--0.5 N NaOH) at elevated temperatures can be used to extract DNA from small pieces of tissue. RNA is destroyed by the treatment. In tissues which have been previously exposed to tritiated thymidine, aliquots from the extracting solution can be used directly for the determination of DNA by ethidium bromide fluorescence and radioactivity in both DNA and the nucleotide pool.

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Year:  1979        PMID: 41020     DOI: 10.1111/1523-1747.ep12550415

Source DB:  PubMed          Journal:  J Invest Dermatol        ISSN: 0022-202X            Impact factor:   8.551


  3 in total

1.  Fluorometric determination of DNA in epidermis and cultured fibroblasts, using 4'-6-diamidino-2-phenylindole (DAPI).

Authors:  J C Meyer; H Grundmann
Journal:  Arch Dermatol Res       Date:  1984       Impact factor: 3.017

2.  Cutaneous cytodestructive potency of lignans. I. A comparative evaluation of influence on epidermal and dermal DNA synthesis and on dermal microcirculation in the hairless mouse.

Authors:  G von Krogh; H I Maibach
Journal:  Arch Dermatol Res       Date:  1982       Impact factor: 3.017

3.  Retinoids increase the response of guinea pig but not human keratinocytes to agonists of adenylate cyclase in vitro.

Authors:  D I Wilkinson; E K Orenberg
Journal:  Arch Dermatol Res       Date:  1983       Impact factor: 3.017

  3 in total

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