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Literature DB >> 4101522

The M band. Studies with fluorescent antibody staining.

Abstract

The M band can be extracted from fibrils suspended in 5 mM Tris buffer, pH 8.0, for 15 min. The M band is completely removed only from fibrils of sarcomere lengths greater than 2.1 micro. Extraction does not alter the fluorescent antimyosin staining pattern of the A band, thus providing strong evidence that no alteration of the structural integrity of the thick filament has occurred. Fluorescent antibody staining of the M band of unextracted fibrils can be prevented specifically by absorbing the fluorescent antibody with extracted M band material prior to staining. This verifies the specificity of the extraction procedure.

Entities: Chemical

Mesh：

Substances：

Year: 1971 PMID： 4101522 PMCID： PMC2108176 DOI： 10.1083/jcb.48.2.340

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

10 in total

1. Extraction of proteins other than myosin from the isolated rabbit myofibril.

Authors: S V PERRY; A CORSI
Journal: Biochem J Date: 1958-01 Impact factor: 3.857

2. The myosin filament. I. Structural organization from antibody staining observed in electron microscopy.

Authors: F A Pepe
Journal: J Mol Biol Date: 1967-07-28 Impact factor: 5.469

Review 3. Analysis of antibody staining patterns obtained with striated myofibrils in fluorescence microscopy and electron microscopy.

Authors: F A Pepe
Journal: Int Rev Cytol Date: 1968

4. The myosin filament. II. Interaction between myosin and actin filaments observed using antibody staining in fluorescent and electron microscopy.

Authors: F A Pepe
Journal: J Mol Biol Date: 1967-07-28 Impact factor: 5.469

5. "M-substance", a new protein constituting the M-line of myofibrils.

Authors: T Masaki; O Takaiti; S Ebashi
Journal: J Biochem Date: 1968-12 Impact factor: 3.387

6. X-ray diffraction from living striated muscle during contraction.

Authors: G F Elliott; J Lowy; B M Millman
Journal: Nature Date: 1965-06-26 Impact factor: 49.962

7. Electron microscope observations of the location of actin and tropomyosin in the rabbit myofibril.

Authors: A Corsi; U Muscatello; I Ronchetti
Journal: J Ultrastruct Res Date: 1967-08

8. The effect of various protein fractions on Z- and M-line reconstitution.

Authors: M H Stromer; D J Hartshorne; H Mueller; R V Rice
Journal: J Cell Biol Date: 1969-01 Impact factor: 10.539

9. The distribution of muscle antigens in contracted myofibrils determined by fluorescein-labeled antibodies.

Authors: B TUNIK; H HOLTZER
Journal: J Biophys Biochem Cytol Date: 1961-10

10. Some aspects of the structural organization of the myofibril as revealed by antibody--staining methods.

Authors: F A Pepe
Journal: J Cell Biol Date: 1966-03 Impact factor: 10.539

10 in total

12 in total

1. Monovalent antibodies against MM-creatine kinase remove the M line from myofibrils.

Authors: T Wallimann; G Pelloni; D C Turner; H M Eppenberger
Journal: Proc Natl Acad Sci U S A Date: 1978-09 Impact factor: 11.205

2. Comparison of the localization of several muscle proteins in relaxed and contracted myofibrils.

Authors: C W Heizmann; I E Bläuenstein; H M Eppenberger
Journal: Experientia Date: 1978-01-15

3. Isolation and immunofluorescent localization of actin derived from rat skeletal muscle.

Authors: H M Abandowitz; P K Basrur
Journal: Histochemie Date: 1973

4. Monoclonal antibodies against myofibrillar components of rat skeletal muscle decorate the intermediate filaments of cultured cells.

Authors: J J Lin
Journal: Proc Natl Acad Sci U S A Date: 1981-04 Impact factor: 11.205

5. Myosin types in human skeletal muscle fibers.

Authors: R Billeter; H Weber; H Lutz; H Howald; H M Eppenberger; E Jenny
Journal: Histochemistry Date: 1980

6. Myofibrillar M-band proteins represent constituents of native thick filaments, frayed filaments and bare zone assemblages.

Authors: M Bähler; T Wallimann; H M Eppenberger
Journal: J Muscle Res Cell Motil Date: 1985-12 Impact factor: 2.698