Literature DB >> 4096388

Determination of Sepharose-bound protein with Coomassie brilliant blue G-250.

R A Asryants, I V Duszenkova, N K Nagradova.   

Abstract

The colorimetric procedure of Bradford (M.M. Bradford, 1976, Anal. Biochem. 72, 248-254) was found to be convenient for determining the content of a protein immobilized on Sepharose. Being simple, sensitive, and rapid, this method appears very useful in studies involving multiple analyses of immobilized protein species present at low concentrations.

Entities:  

Mesh：

Substances：

Year:  1985        PMID： 4096388     DOI： 10.1016/0003-2697(85)90222-2

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  10 in total

1.  Introduction of a disulfide bridge enhances the thermostability of a Streptomyces olivaceoviridis xylanase mutant.

Authors:  H M Yang; B Yao; K Meng; Y R Wang; Y G Bai; N F Wu
Journal:  J Ind Microbiol Biotechnol       Date:  2006-12-01       Impact factor: 3.346

2.  Misfolded forms of glyceraldehyde-3-phosphate dehydrogenase interact with GroEL and inhibit chaperonin-assisted folding of the wild-type enzyme.

Authors:  Oxana V Polyakova; Olivier Roitel; Regina A Asryants; Alexei A Poliakov; Guy Branlant; Vladimir I Muronetz
Journal:  Protein Sci       Date:  2005-03-01       Impact factor: 6.725

3.  The regulatory beta subunit of phosphorylase kinase interacts with glyceraldehyde-3-phosphate dehydrogenase.

Authors:  Igor G Boulatnikov; Owen W Nadeau; Patrick J Daniels; Jessica M Sage; Marina D Jeyasingham; Maria T Villar; Antonio Artigues; Gerald M Carlson
Journal:  Biochemistry       Date:  2008-06-13       Impact factor: 3.162

4.  Reversible dissociation of the catalytically active subunits of pigeon liver malic enzyme.

Authors:  G G Chang; T M Huang; T C Chang
Journal:  Biochem J       Date:  1988-08-15       Impact factor: 3.857

5.  The Hsp90 inhibitor 17-AAG represses calcium-induced cytokeratin 1 and 10 expression in HaCaT keratinocytes.

Authors:  Sadanori Miyoshi; Shota Yamazaki; Asato Uchiumi; Yohtaro Katagata
Journal:  FEBS Open Bio       Date:  2012-03-25       Impact factor: 2.693

Review 6.  Brilliant Blue Dyes in Daily Food: How Could Purinergic System Be Affected?

Authors:  Leonardo Gomes Braga Ferreira; Robson Xavier Faria; Natiele Carla da Silva Ferreira; Rômulo José Soares-Bezerra
Journal:  Int J Food Sci       Date:  2016-10-19

7.  Synthesis, characterization and in vitro inhibition of metal complexes of pyrazole based sulfonamide on human erythrocyte carbonic anhydrase isozymes I and II.

Authors:  Nurgün Büyükkıdan; Bülent Büyükkıdan; Metin Bülbül; Rahmi Kasımoğulları; Samet Mert
Journal:  J Enzyme Inhib Med Chem       Date:  2017-12       Impact factor: 5.051

8.  Purification and characterization of a thaumatin-like protein-1 with polyphenol oxidase activity found in Prunus mume.

Authors:  Yuan Ma; Ming Luo; Yingping Xu; Yingjia Liu; Xiaocui Liu; Xiufang Bi; Yiping Yuan; Fan Su; Xiaocui Yin
Journal:  RSC Adv       Date:  2020-08-04       Impact factor: 4.036

9.  Adventitious root primordia formation and development in the stem of Ananas comosus var. bracteatus slip.

Authors:  Mark Owusu Adjei; Yixuan Xiang; Yehua He; Xuzixin Zhou; Meiqin Mao; Jiawen Liu; Hao Hu; Jiaheng Luo; Huiling Zhang; Lijun Feng; Wei Yang; Xi Li; Jun Ma
Journal:  Plant Signal Behav       Date:  2021-07-21

10.  Effects of Quercetin on the Growth and Expression of Immune-Pathway-Related Genes in Silkworm (Lepidoptera: Bombycidae).

Authors:  Guiqin Shi; Zhaoyang Kang; Fei Ren; Yuan Zhou; Penglei Guo
Journal:  J Insect Sci       Date:  2020-11-01       Impact factor: 1.857
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.