Literature DB >> 4093482

Rapid purification of host-specific pathotoxins from Alternaria alternata f. sp. lycopersici by solid-phase adsorption on octadecylsilane.

S D Clouse, A N Martensen, D G Gilchrist.   

Abstract

A simplified, rapid procedure for the purification of two phytotoxic metabolites (TA and TB) from cell-free culture filtrates of Alternaria alternata f. sp. lycopersici was developed using hydrophobic chromatography on macroporous, C18 bonded silica followed by gel filtration. Baseline separation of TA from TB was achieved in the final preparation and yields of 80% were obtained with retention of full biological activity in all assay systems tested. Toxin analysis by nuclear magnetic resonance and high-performance liquid chromatography indicated TA was at least 99% pure. The efficiency of the procedure allows rapid accumulation of pure TA and TB for studies on the molecular mode of action and host plant response to this novel host-specific phytotoxin.

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Year:  1985        PMID： 4093482     DOI： 10.1016/s0021-9673(01)93524-1

Source DB:  PubMed          Journal:  J Chromatogr


  10 in total

1.  Multiple epoxide hydrolases in Alternaria alternata f. sp. lycopersici and their relationship to medium composition and host-specific toxin production.

Authors:  C Morisseau; B L Ward; D G Gilchrist; B D Hammock
Journal:  Appl Environ Microbiol       Date:  1999-06       Impact factor: 4.792

2.  Effects of Alternaria alternata f.sp. lycopersici toxins on pollen.

Authors:  R J Bino; J Franken; H M Witsenboer; J Hille; J J Dons
Journal:  Theor Appl Genet       Date:  1988-08       Impact factor: 5.699

3.  Production of the Mycotoxin Fumonisin B(1) by Alternaria alternata f. sp. lycopersici.

Authors:  J Chen; C J Mirocha; W Xie; L Hogge; D Olson
Journal:  Appl Environ Microbiol       Date:  1992-12       Impact factor: 4.792

4.  Expression of the antiapoptotic baculovirus p35 gene in tomato blocks programmed cell death and provides broad-spectrum resistance to disease.

Authors:  James E Lincoln; Craig Richael; Bert Overduin; Kathy Smith; Richard Bostock; David G Gilchrist
Journal:  Proc Natl Acad Sci U S A       Date:  2002-10-25       Impact factor: 11.205

5.  A longevity assurance gene homolog of tomato mediates resistance to Alternaria alternata f. sp. lycopersici toxins and fumonisin B1.

Authors:  B F Brandwagt; L A Mesbah; F L Takken; P L Laurent; T J Kneppers; J Hille; H J Nijkamp
Journal:  Proc Natl Acad Sci U S A       Date:  2000-04-25       Impact factor: 11.205

6.  Toxicity of the mycotoxins fumonisins B1 and B2 and Alternaria alternata f. sp. lycopersici toxin (AAL) in cultured mammalian cells.

Authors:  W T Shier; H K Abbas; C J Mirocha
Journal:  Mycopathologia       Date:  1991-11       Impact factor: 2.574

7.  Inheritance and genetic mapping of resistance to Alternaria alternata f. sp. lycopersici in Lycopersicon pennellii.

Authors:  E A van der Biezen; T Glagotskaya; B Overduin; H J Nijkamp; J Hille
Journal:  Mol Gen Genet       Date:  1995-05-20

8.  Alanine is a precursor in the biosynthesis of fumonisin B1 by Fusarium moniliforme.

Authors:  B E Branham; R D Plattner
Journal:  Mycopathologia       Date:  1993-11       Impact factor: 2.574

9.  Sphingolipids and plant defense/disease: the "death" connection and beyond.

Authors:  Robert Berkey; Dipti Bendigeri; Shunyuan Xiao
Journal:  Front Plant Sci       Date:  2012-04-10       Impact factor: 5.753

10.  A Natural Variation of Fumonisin Gene Cluster Associated with Fumonisin Production Difference in Fusarium fujikuroi.

Authors:  Sharmin Sultana; Miha Kitajima; Hironori Kobayashi; Hiroyuki Nakagawa; Masafumi Shimizu; Koji Kageyama; Haruhisa Suga
Journal:  Toxins (Basel)       Date:  2019-04-03       Impact factor: 4.546
  10 in total

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