Mass spectrometric identification of the pentasialoganglioside GP1c of embryonic chicken brain.

Gangliosides were extracted from 11-day-old chicken embryos and finally purified by chromatography on high performance thin-layer plates. Four fractions migrating more slowly than ganglioside GQ1b were obtained by preparative thin-layer chromatography. With the aid of negative ion fast atom bombardment mass spectrometry, one of these could be identified as GP1c.