Literature DB >> 4091804

Purification of fish muscle myosin heavy chain and quantification of the specific polyribosome-bound polypeptide.

E Lied, A von der Decken.   

Abstract

A purification procedure for fish myosin heavy chain is described. The protein was injected into rabbits for production of antibodies. The specificity of the antibodies was determined by immunoblotting. The enzyme-linked immunosorbent assay technique was applied to quantify myosin heavy chain bound to isolated polyribosomes of epaxial muscle from fish.

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Year:  1985        PMID： 4091804      PMCID： PMC1152903          DOI： 10.1042/bj2320467

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  18 in total

1.  PREPARATION AND PROPERTIES OF PURIFIED COD MYOSIN.

Authors:  I M MACKIE; J J CONNELL
Journal:  Biochim Biophys Acta       Date:  1964-12-09

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

4.  Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.

Authors:  E Engvall; P Perlmann
Journal:  J Immunol       Date:  1972-07       Impact factor: 5.422

5.  Paramyosin and the filaments of molluscan "catch" muscles. II. Native filaments: isolation and characterization.

Authors:  A G Szent-Györgyi; C Cohen; J Kendrick-Jones
Journal:  J Mol Biol       Date:  1971-03-14       Impact factor: 5.469

6.  Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.

Authors:  E Engvall; P Perlmann
Journal:  Immunochemistry       Date:  1971-09

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

Review 8.  Translational control of protein synthesis.

Authors:  H F Lodish
Journal:  Annu Rev Biochem       Date:  1976       Impact factor: 23.643

9.  Protein synthesis in vitro after cryopreservation of rat skeletal muscle.

Authors:  B Lund; A von der Decken
Journal:  Z Tierphysiol Tierernahr Futtermittelkd       Date:  1980

10.  The effect of adenosine triphosphate, inorganic pyrophosphate and inorganic tripolyphosphate on the stability of cod myosin.

Authors:  I M Mackie
Journal:  Biochim Biophys Acta       Date:  1966-01-25
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  2 in total

1.  Dietary protein levels affect growth and protein metabolism in trunk muscle of cod, Gadus morhua.

Authors:  A von der Decken; E Lied
Journal:  J Comp Physiol B       Date:  1992       Impact factor: 2.200

2.  Myosin heavy chain synthesis in white trunk muscle of cod (Gadus morhua) fed different ration sizes.

Authors:  A von der Decken; E Lied
Journal:  Fish Physiol Biochem       Date:  1989-11       Impact factor: 2.794
  2 in total

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