Literature DB >> 4091282

Enhanced chemiluminescent method for the detection of DNA dot-hybridization assays.

J A Matthews, A Batki, C Hynds, L J Kricka.   

Abstract

A simple enhanced chemiluminescent procedure for the quantitation of DNA hybridization to dot blots is described. The method utilizes DNA probes labeled with biotin, which are detected using a biotinylated streptavidin-horseradish peroxidase complex. The peroxidase enzyme then takes part in an enhanced chemiluminescent reaction with luminol, peroxide, and an enhancer. The method can be used to give quantitative results using a photomultiplier tube or qualitative results by recording the light emission on instant photographic film.

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Year:  1985        PMID： 4091282     DOI： 10.1016/0003-2697(85)90073-9

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  17 in total

1.  Fesselin: a novel synaptopodin-like actin binding protein from muscle tissue.

Authors:  B D Leinweber; R S Fredricksen; D R Hoffman; J M Chalovich
Journal:  J Muscle Res Cell Motil       Date:  1999-08       Impact factor: 2.698

2.  The cyclin-dependent kinase inhibitor p21 (WAF1) is required for survival of differentiating neuroblastoma cells.

Authors:  W Poluha; D K Poluha; B Chang; N E Crosbie; C M Schonhoff; D L Kilpatrick; A H Ross
Journal:  Mol Cell Biol       Date:  1996-04       Impact factor: 4.272

3.  Preparation of europium-labelled DNA probes and their properties.

Authors:  P Hurskainen; P Dahlén; J Ylikoski; M Kwiatkowski; H Siitari; T Lövgren
Journal:  Nucleic Acids Res       Date:  1991-03-11       Impact factor: 16.971

Review 4.  Diagnostic deoxyribonucleic acid probes for infectious diseases.

Authors:  F C Tenover
Journal:  Clin Microbiol Rev       Date:  1988-01       Impact factor: 26.132

5.  Monoadduct forming photochemical reagents for labeling nucleic acids for hybridization.

Authors:  J P Albarella; R L Minegar; W L Patterson; N Dattagupta; E Carlson
Journal:  Nucleic Acids Res       Date:  1989-06-12       Impact factor: 16.971

Review 6.  Luminol-based chemiluminescent signals: clinical and non-clinical application and future uses.

Authors:  Parvez Khan; Danish Idrees; Michael A Moxley; John A Corbett; Faizan Ahmad; Guido von Figura; William S Sly; Abdul Waheed; Md Imtaiyaz Hassan
Journal:  Appl Biochem Biotechnol       Date:  2014-04-22       Impact factor: 2.926

7.  Thin filament activation probed by fluorescence of N-((2-(iodoacetoxy)ethyl)-N-methyl)amino-7-nitrobenz-2-oxa-1,3-diazole-labeled troponin I incorporated into skinned fibers of rabbit psoas muscle.

Authors:  B Brenner; T Kraft; L C Yu; J M Chalovich
Journal:  Biophys J       Date:  1999-11       Impact factor: 4.033

8.  Catalytic and immunochemical detection of hepatic and extrahepatic microsomal cytochrome P450 1A1 (CYP1A1) in white-sided dolphin (Lagenorhynchus acutus).

Authors:  Joanna Y Wilson; Michael J Moore; John J Stegeman
Journal:  Aquat Toxicol       Date:  2009-11-04       Impact factor: 4.964

9.  Quantitative assay of PCR-amplified hepatitis B virus DNA using a peroxidase-labelled DNA probe and enhanced chemiluminescence.

Authors:  A Erhardt; S Schaefer; N Athanassiou; M Kann; W H Gerlich
Journal:  J Clin Microbiol       Date:  1996-08       Impact factor: 5.948

10.  Effects of angiopoietins-1 and -2 on the receptor tyrosine kinase Tie2 are differentially regulated at the endothelial cell surface.

Authors:  Tania M Hansen; Harprit Singh; Tariq A Tahir; Nicholas P J Brindle
Journal:  Cell Signal       Date:  2010-03       Impact factor: 4.315
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