Literature DB >> 4091272

Separation of bacterial luciferase from oxidoreductases by affinity chromatography.

T S Tsai.   

Abstract

The NADH-dependent and NADPH-dependent oxidoreductase activities associated with bacterial luciferase in Vibrio (Beneckea) harveyi can simultaneously be removed from purified luciferase through a Blue Sepharose CL-6B column. The result achieved with this one-step affinity chromatography is similar to that obtained with two sequential "reverse-affinity" chromatographies.

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Year:  1985        PMID: 4091272     DOI: 10.1016/0003-2697(85)90063-6

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  1 in total

1.  Bioluminescence of the insect pathogen Xenorhabdus luminescens.

Authors:  T M Schmidt; K Kopecky; K H Nealson
Journal:  Appl Environ Microbiol       Date:  1989-10       Impact factor: 4.792

  1 in total

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