Literature DB >> 4091265

A method for the efficient blotting of strongly basic proteins from sodium dodecyl sulfate-polyacrylamide gels to nitrocellulose.

B Szewczyk, L M Kozloff.   

Abstract

An improved procedure for the electrophoretic transfer of strongly basic proteins from sodium dodecyl sulfate-polyacrylamide gels to nitrocellulose is described. The use of more alkaline transfer buffers and the omission of an equilibration step before the transfer allow for the almost complete transfer of strongly basic proteins from gels to nitrocellulose without lowering the transfer efficiency for other proteins.

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Year:  1985        PMID： 4091265     DOI： 10.1016/0003-2697(85)90528-7

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  34 in total

1.  Monoclonal antibodies to outer membrane protein PII block interactions of Neisseria gonorrhoeae with human neutrophils.

Authors:  C Elkins; R F Rest
Journal:  Infect Immun       Date:  1990-04       Impact factor: 3.441

2.  Denitration of peroxynitrite-treated proteins by 'protein nitratases' from rat brain and heart.

Authors:  W N Kuo; R N Kanadia; V P Shanbhag; R Toro
Journal:  Mol Cell Biochem       Date:  1999-11       Impact factor: 3.396

3.  Anaerobic growth of gonococci does not alter their Opa-mediated interactions with human neutrophils.

Authors:  J V Frangipane; R F Rest
Journal:  Infect Immun       Date:  1992-05       Impact factor: 3.441

4.  The ferredoxin-thioredoxin reductase variable subunit gene from Anacystis nidulans.

Authors:  M Szekeres; M Droux; B B Buchanan
Journal:  J Bacteriol       Date:  1991-03       Impact factor: 3.490

5.  Interactions of Neisseria gonorrhoeae with human neutrophils: studies with purified PII (Opa) outer membrane proteins and synthetic Opa peptides.

Authors:  F L Naids; B Belisle; N Lee; R F Rest
Journal:  Infect Immun       Date:  1991-12       Impact factor: 3.441

6.  Cytosolic rat brain synapsin I is a diacylglycerol kinase.

Authors:  D W Kahn; J M Besterman
Journal:  Proc Natl Acad Sci U S A       Date:  1991-07-15       Impact factor: 11.205

7.  Cell-mediated and humoral immune responses after vaccination of human volunteers with the live vaccine strain of Francisella tularensis.

Authors:  D M Waag; K T McKee; G Sandstrom; L L Pratt; C R Bolt; M J England; G O Nelson; J C Williams
Journal:  Clin Diagn Lab Immunol       Date:  1995-03

8.  Oligomerization of the bacteriophage lambda S protein in the inner membrane of Escherichia coli.

Authors:  M T Zagotta; D B Wilson
Journal:  J Bacteriol       Date:  1990-02       Impact factor: 3.490

9.  Identification of a second RcsA protein, a positive regulator of colanic acid capsular polysaccharide genes, in Escherichia coli.

Authors:  K P Dierksen; J E Trempy
Journal:  J Bacteriol       Date:  1996-08       Impact factor: 3.490

10.  Phylogenetically conserved amino acids of MBP and P0 from amphibian myelin.

Authors:  J Karthigasan; T K Bauer; D B Teplow; R A Saavedra; D A Kirschner
Journal:  J Mol Neurosci       Date:  1992       Impact factor: 3.444
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