A model of experimental lymphedema in rats' limbs.

The authors' modified Olszewski method (J Cardiovasc Surg 9:178-183, 1968) for preparing chronic lymphedema with rats' limbs is presented. The incidence of chronic lymphedema formation after 9 months of follow-up was 64%. The experiment demonstrates that there is a brisk regeneration of the lymphatics under the lymphedema. The regeneration observed took place on the lateral wall of the blocked lymphatics, except for regeneration of capillary lymphatics, and the newborn lymphatics grew about 1 mm each day. There are indeed preexisting lymphatics in the body. They are of significant importance in the establishment of the collateral circulation because of their great number and early opening. Lymphatic regeneration and opening could be prevented by tissue scarring. Effective prevention of the formation of tissue scarring has important significance in preventing the development of chronic lymphedema.