Gas chromatography of amino acids in urine and haemofiltrate.

Capillary gas chromatography of amino acid derivatives obtained from biological material shows a large number of previously unknown constituents. The profiles of amino acids obtained from urine of healthy individuals and haemofiltrate of uraemic patients indicate that haemofiltration removes some amino acids to a considerably higher extent from uraemic patients than the kidney does from healthy persons. For instance, if haemofiltration is required three times a week, an approximately ten-fold amount of the essential amino acid methionine and a forty-fold amount of the essential amino acid leucine is lost compared to their excretion in urine by a healthy individual over the same period.