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Literature DB >> 408374

Modified indirect fluorescent-antibody test for aspergillosis.

Abstract

Diffuse fluorescence in an indirect fluorescent-antibody test for antibody to Aspergillus fumigatus was eliminated by using germlings, rather than hyphae, as antigen. The modified method yielded diagnostic titers larger than or equal to 1:32 in 24 of 25 known cases, was highly specific, and showed good correlation with clinical status and results of immunodiffusion. This technique, which uses the germling antigen, inactivation of test serum at 59 +/- 1 degrees C, and anti-immunoglobulin G conjugate, is a sensitive, specific, and reliable quantitative indicator of aspergillosis and is useful in following the course of the disease.

Entities: Disease Species

Mesh：

Substances：
Antibodies, Fungal

Year: 1977 PMID： 408374 PMCID： PMC274725 DOI： 10.1128/jcm.6.2.161-165.1977

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

11 in total

1. Indirect immunofluorescence test for the detection of Aspergillus fumigatus antibodies.

Authors: D W Warnock
Journal: J Clin Pathol Date: 1974-11 Impact factor: 3.411

Review 2. The current status of serologic, immunologic and skin tests in the diagnosis of pulmonary mycoses. Report of the Committee on Fungus Diseases and Subcommittee on Criteria for Clinical Diagnosis--American College of Chest Physicians.

Authors:
Journal: Chest Date: 1973-02 Impact factor: 9.410

3. Pulmonary aspergillosis in sanatoriums in the south central United States. A national communicable disease center cooperative mycoses study.

Authors: J D Parker; G A Sarosi; I L Doto; F E Tosh
Journal: Am Rev Respir Dis Date: 1970-04

Review 5. A guide to the recent literature on aspergillosis as caused by Aspergillus fumigatus, a fungus frequently found in self-heating organic matter.

Authors: P B Marsh; P D Millner; J M Kla
Journal: Mycopathologia Date: 1979-11-30 Impact factor: 2.574

Review 6. Immunodiagnosis of aspergillosis.

Authors: V P Kurup; A Kumar
Journal: Clin Microbiol Rev Date: 1991-10 Impact factor: 26.132

6 in total

Modified indirect fluorescent-antibody test for aspergillosis.

1. Indirect immunofluorescence test for the detection of Aspergillus fumigatus antibodies.

Review 2. The current status of serologic, immunologic and skin tests in the diagnosis of pulmonary mycoses. Report of the Committee on Fungus Diseases and Subcommittee on Criteria for Clinical Diagnosis--American College of Chest Physicians.

3. Pulmonary aspergillosis in sanatoriums in the south central United States. A national communicable disease center cooperative mycoses study.

4. Immunoglobulin classes of antibodies to Aspergillus fumigatus in patients with pulmonary aspergillosis.

5. Diagnostic mycoserology by immunoelectroosmophoresis: a general, rapid, and sensitive microtechnic.

6. [Current aspects of deep mycoses. New acquisitions in mycological and immunological diagnosis of mycotic infections].

7. Fluorescent antibody test for the serological diagnosis of gonorrhea.

8. An aspergillus immunodiffusion test in the early diagnosis of aspergillosis in adult leukemia patients.

9. Use of the immunodiffusion test in the serodiagnosis of aspergillosis.

10. APPLICATION OF FLUORESCENT-ANTIBODY PROCEDURES TO THE STUDY OF PATHOGENIC DEMATIACEOUS FUNGI.

1. Detection of IgG and IgE antibodies to Aspergillus fumigatus in human sera by immunogold assay.

2. Immunoblot analysis of serological responses in invasive aspergillosis.

3. An 88-kilodalton antigen secreted by Aspergillus fumigatus.

4. Electron microscopic examination of ribosome preparations from germinated spores of Aspergillus fumigatus.

Review 5. A guide to the recent literature on aspergillosis as caused by Aspergillus fumigatus, a fungus frequently found in self-heating organic matter.

Review 6. Immunodiagnosis of aspergillosis.