Literature DB >> 4083486

Sensitive spectrophotometric method for the quantitative estimation of collagen.

M Marotta, G Martino.   

Abstract

A method to estimate collagen concentration is described. It is based on the ability of the dye Sirius Red F3BA to quantitatively precipitate collagen in acetic acid solution. Collagen content is determined by evaluating spectrophotometrically the absorbance decrease of a Sirius Red/acetic acid solution. The assay can be used in the range from 1 to 50 micrograms protein. The method is specific, sensitive, simple, and rapid.

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Year:  1985        PMID: 4083486     DOI: 10.1016/0003-2697(85)90443-9

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  14 in total

1.  Polymerization and matrix physical properties as important design considerations for soluble collagen formulations.

Authors:  S T Kreger; B J Bell; J Bailey; E Stites; J Kuske; B Waisner; S L Voytik-Harbin
Journal:  Biopolymers       Date:  2010-08       Impact factor: 2.505

2.  Effects of early and late chronic pressure overload on extracellular matrix remodeling.

Authors:  Jing Lin; Harrison B Davis; Qiuxia Dai; Youn-Min Chou; Teresa Craig; Carmen Hinojosa-Laborde; Merry L Lindsey
Journal:  Hypertens Res       Date:  2008-06       Impact factor: 3.872

3.  Thermal memory in self-assembled collagen fibril networks.

Authors:  Martijn de Wild; Wim Pomp; Gijsje H Koenderink
Journal:  Biophys J       Date:  2013-07-02       Impact factor: 4.033

4.  Protective effect of Syzygium cumini against pesticide-induced cardiotoxicity.

Authors:  Neha Atale; Khushboo Gupta; Vibha Rani
Journal:  Environ Sci Pollut Res Int       Date:  2014-03-23       Impact factor: 4.223

5.  Myocardial stiffness in patients with heart failure and a preserved ejection fraction: contributions of collagen and titin.

Authors:  Michael R Zile; Catalin F Baicu; John S Ikonomidis; Robert E Stroud; Paul J Nietert; Amy D Bradshaw; Rebecca Slater; Bradley M Palmer; Peter Van Buren; Markus Meyer; Margaret M Redfield; David A Bull; Henk L Granzier; Martin M LeWinter
Journal:  Circulation       Date:  2015-01-30       Impact factor: 29.690

Review 6.  Review of collagen I hydrogels for bioengineered tissue microenvironments: characterization of mechanics, structure, and transport.

Authors:  Elizabeth E Antoine; Pavlos P Vlachos; Marissa Nichole Rylander
Journal:  Tissue Eng Part B Rev       Date:  2014-07-22       Impact factor: 6.389

7.  SHARPIN regulates collagen architecture and ductal outgrowth in the developing mouse mammary gland.

Authors:  Emilia Peuhu; Riina Kaukonen; Martina Lerche; Markku Saari; Camilo Guzmán; Pia Rantakari; Nicola De Franceschi; Anni Wärri; Maria Georgiadou; Guillaume Jacquemet; Elina Mattila; Reetta Virtakoivu; Yuming Liu; Youmna Attieh; Kathleen A Silva; Timo Betz; John P Sundberg; Marko Salmi; Marie-Ange Deugnier; Kevin W Eliceiri; Johanna Ivaska
Journal:  EMBO J       Date:  2016-12-14       Impact factor: 11.598

8.  Progressive induction of left ventricular pressure overload in a large animal model elicits myocardial remodeling and a unique matrix signature.

Authors:  William M Yarbrough; Rupak Mukherjee; Robert E Stroud; William T Rivers; J Marshall Oelsen; Jennifer A Dixon; Shaina R Eckhouse; John S Ikonomidis; Michael R Zile; Francis G Spinale
Journal:  J Thorac Cardiovasc Surg       Date:  2011-11-04       Impact factor: 5.209

9.  Hyaluronan concentration within a 3D collagen matrix modulates matrix viscoelasticity, but not fibroblast response.

Authors:  S T Kreger; S L Voytik-Harbin
Journal:  Matrix Biol       Date:  2009-05-13       Impact factor: 11.583

10.  The effects of early or late treatment of osteochondral defects on joint homoeostasis: an experimental study in rabbits.

Authors:  Mehmet Hakan Ozsoy; Semih Aydogdu; Dilek Taskiran; Murat Sezak; Mutlu Hayran; Fikri Oztop; Arzu Ozsoy
Journal:  Knee Surg Sports Traumatol Arthrosc       Date:  2008-12-16       Impact factor: 4.342

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