| Literature DB >> 4083486 |
Abstract
A method to estimate collagen concentration is described. It is based on the ability of the dye Sirius Red F3BA to quantitatively precipitate collagen in acetic acid solution. Collagen content is determined by evaluating spectrophotometrically the absorbance decrease of a Sirius Red/acetic acid solution. The assay can be used in the range from 1 to 50 micrograms protein. The method is specific, sensitive, simple, and rapid.Entities:
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Year: 1985 PMID: 4083486 DOI: 10.1016/0003-2697(85)90443-9
Source DB: PubMed Journal: Anal Biochem ISSN: 0003-2697 Impact factor: 3.365