Literature DB >> 4083279

The genetic polymorphism of alpha 2-HS glycoprotein: study by ultrathin-layer isoelectric focusing and immunoblot.

B Boutin, S H Feng, P Arnaud.   

Abstract

The genetic polymorphism of human alpha 2-HS glycoprotein was studied by ultrathin-layer isoelectric focusing in a pH range 4.2-4.9 followed by immunoblotting using antisera specific for the protein. Three common phenotypes, HSGA1-1, HSGA2-2, and HSGA1-2, were easily recognized using this technique. Each homozygous phenotype was constituted by five major bands with different pI's, and the heterozygous phenotype represented an exact combination of each of them. After removal of sialic acid, each homozygous pattern was resumed in a single band, which differed in charge between HSGA1 and HSGA2. Asialo-heterozygous HSG1-2 combined the two bands characteristic of each allele product. Family studies are in agreement with an autosomal codominant transmission of the two alleles. Population studies indicate that the frequency of the HSGA1 and 2 alleles are .65 and .35, respectively.

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Year:  1985        PMID： 4083279      PMCID： PMC1684736     

Source DB:  PubMed          Journal:  Am J Hum Genet        ISSN： 0002-9297            Impact factor:   11.025


  23 in total

1.  Changes in the serum alpha glycoprotein distribution in trauma patients.

Authors:  C J van Oss; P M Bronson; J R Border
Journal:  J Trauma       Date:  1975-05

2.  A serum DNA-binding protein absent in malignant disease.

Authors:  J G Lewis; C M André
Journal:  FEBS Lett       Date:  1978-08-15       Impact factor: 4.124

3.  Origin of plasma alpha2HS-glycoprotein and its accumulation in bone.

Authors:  J T Triffitt; U Gebauer; B A Ashton; M E Owen; J J Reynolds
Journal:  Nature       Date:  1976-07-15       Impact factor: 49.962

4.  Microheterogeneity of serum transferrin, haptoglobin and alpha 2 HS glycoprotein examined by high resolution two-dimensional electrophoresis.

Authors:  N L Anderson; N G Anderson
Journal:  Biochem Biophys Res Commun       Date:  1979-05-14       Impact factor: 3.575

5.  Properties of isolated human alpha1-antitrypsins of Pi types M, S and Z.

Authors:  J O Jeppsson; C B Laurell; M Fagerhol
Journal:  Eur J Biochem       Date:  1978-02-01

6.  Ultrathin-layer isoelectric focusing in polyacrylamide gels on cellophane.

Authors:  A Görg; W Postel; R Westermeier
Journal:  Anal Biochem       Date:  1978-08-15       Impact factor: 3.365

7.  Purification of the human plasma alpha2-SH glycoprotein by zinc chelate affinity chromatography.

Authors:  J P Lebreton
Journal:  FEBS Lett       Date:  1977-08-15       Impact factor: 4.124

8.  Isolation of human serum alpha-2 HS glycoprotein.

Authors:  C J van Oss; P M Bronson
Journal:  Prep Biochem       Date:  1974

9.  Serum concentration of human alpha 2 HS glycoprotein during the inflammatory process: evidence that alpha 2 HS glycoprotein is a negative acute-phase reactant.

Authors:  J P Lebreton; F Joisel; J P Raoult; B Lannuzel; J P Rogez; G Humbert
Journal:  J Clin Invest       Date:  1979-10       Impact factor: 14.808

10.  Neuraminidase treatment reveals sialic acid differences in certain genetic variants of the Gc system (vitamin-D-binding protein).

Authors:  H Cleve; W Patutschnick
Journal:  Hum Genet       Date:  1979-03-12       Impact factor: 4.132
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  7 in total

1.  Genetic polymorphism of alpha-2-HS glycoprotein in Lombardy (Italy).

Authors:  N Cerri; F De Ferrari
Journal:  Int J Legal Med       Date:  1991-03       Impact factor: 2.686

2.  Characterization of the molecular basis of the alpha 1-antitrypsin F allele.

Authors:  H Okayama; M Brantly; M Holmes; R G Crystal
Journal:  Am J Hum Genet       Date:  1991-06       Impact factor: 11.025

3.  Characterization of the normal alpha 1-antitrypsin allele Vmunich: a variant associated with a unique protein isoelectric focusing pattern.

Authors:  M D Holmes; M L Brantly; D T Curiel; S Weidinger; R G Crystal
Journal:  Am J Hum Genet       Date:  1990-04       Impact factor: 11.025

4.  Genetic polymorphism of desialyzed alpha 2 HS-glycoprotein by ultrathin isoelectric focusing.

Authors:  I M Sebetan; M M Heshmat
Journal:  Z Rechtsmed       Date:  1988

5.  Alpha 2-HS glycoprotein phenotypes and quantitative hormone and bone measures in postmenopausal women.

Authors:  J E Eichner; C A Friedrich; J A Cauley; M I Kamboh; J P Gutai; L H Kuller; R E Ferrell
Journal:  Calcif Tissue Int       Date:  1990-12       Impact factor: 4.333

6.  The polymorphism of desialyzed alpha 2HS-glycoprotein (AHS): isoelectric focusing in 2.5 M urea as a method for identification of genetic variants.

Authors:  K Umetsu; I Yuasa; T Suzuki
Journal:  Hum Genet       Date:  1986-08       Impact factor: 4.132

7.  Formal and population genetic studies of AHSG: further data from Galicia.

Authors:  F D Cameselle; C Teixeira; E Parra; B Caeiro
Journal:  Int J Legal Med       Date:  1992       Impact factor: 2.686
  7 in total

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