| Literature DB >> 407987 |
Abstract
A method is described for the isolation and purification of a sulphated glycopeptide from human supragingival calculus. The compound was isolated after using EDTA treatment, 2 M CaCl2 extraction, proteolytic digestion, ethanol precipitation, and finally purified by DEAE cellulose chromatography. It migrated as a single component of cellulose acetate electrophoresis, and chemical and infrared spectral analysis showed the presence of covalently attached sulphate groups. The sulphated glycopeptide was distinguished from being a sulphated glycosaminoglycan.Entities:
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Year: 1977 PMID: 407987 DOI: 10.1007/bf02012761
Source DB: PubMed Journal: Calcif Tissue Res ISSN: 0008-0594