Literature DB >> 4077945

Normal-phase high-performance liquid chromatographic separation of non-derivatized ganglioside mixtures.

G Gazzotti, S Sonnino, R Ghidoni.   

Abstract

A new analytical and semi-preparative high-performance liquid chromatographic method for the separation of a brain ganglioside mixture into individual components is described. Gangliosides were applied to a LiChrosorb-NH2 column and eluted with the solvent system acetonitrile-phosphate buffer at different volume ratios and ionic strengths. The elution profile was monitored by flow-through detection of UV absorbance at 215 nm. The separation of mono- to polysialogangliosides was performed in one step in a total elution time lower than 90 min and with high reproducibility.

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Year:  1985        PMID： 4077945     DOI： 10.1016/s0021-9673(01)92475-6

Source DB:  PubMed          Journal:  J Chromatogr


  9 in total

1.  Apprehending ganglioside diversity: a comprehensive methodological approach.

Authors:  Elodie A Y Masson; Estelle Sibille; Lucy Martine; Fanny Chaux-Picquet; Lionel Bretillon; Olivier Berdeaux
Journal:  J Lipid Res       Date:  2015-07-04       Impact factor: 5.922

2.  Structural characterization and in vivo immunosuppressive activity of neuroblastoma GD2.

Authors:  R Li; D Gage; R McKallip; S Ladisch
Journal:  Glycoconj J       Date:  1996-06       Impact factor: 2.916

3.  Stimulation of a Ca(2+)-dependent protein kinase by GM1 ganglioside in nerve growth factor-treated PC12 cells.

Authors:  B S Hilbush; J M Levine
Journal:  Proc Natl Acad Sci U S A       Date:  1991-07-01       Impact factor: 11.205

4.  Chemical and enzymatic transacylation of amide-linked FA of buttermilk gangliosides.

Authors:  Christopher Beermann; Anne-Katrin Röhrig; Günther Boehm
Journal:  Lipids       Date:  2003-08       Impact factor: 1.880

5.  E-selectin receptors on human leukocytes.

Authors:  Leonardo Nimrichter; Monica M Burdick; Kazuhiro Aoki; Wouter Laroy; Mark A Fierro; Sherry A Hudson; Christopher E Von Seggern; Robert J Cotter; Bruce S Bochner; Michael Tiemeyer; Konstantinos Konstantopoulos; Ronald L Schnaar
Journal:  Blood       Date:  2008-06-25       Impact factor: 22.113

6.  Liquid chromatography-high-resolution mass spectrometry for quantitative analysis of gangliosides.

Authors:  Bertram Fong; Carmen Norris; Edwin Lowe; Paul McJarrow
Journal:  Lipids       Date:  2009-07-26       Impact factor: 1.880

7.  Ceramide structure predicts tumor ganglioside immunosuppressive activity.

Authors:  S Ladisch; R Li; E Olson
Journal:  Proc Natl Acad Sci U S A       Date:  1994-03-01       Impact factor: 11.205

8.  Ganglioside Extraction, Purification and Profiling.

Authors:  Mitchell J Porter; Gao-Lan Zhang; Ronald L Schnaar
Journal:  J Vis Exp       Date:  2021-03-12       Impact factor: 1.355

9.  Specific synthesis of neurostatin and gangliosides O-acetylated in the outer sialic acids using a sialate transferase.

Authors:  Lorenzo Romero-Ramírez; Isabel García-Álvarez; Ramón Campos-Olivas; Michel Gilbert; Marie-France Goneau; Alfonso Fernández-Mayoralas; Manuel Nieto-Sampedro
Journal:  PLoS One       Date:  2012-12-03       Impact factor: 3.240
  9 in total

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