Literature DB >> 4075394

Poly(dG)-poly(dC) sequences, under torsional stress, induce an altered DNA conformation upon neighboring DNA sequences.

T Kohwi-Shigematsu, Y Kohwi.   

Abstract

Supercoiled plasmid DNAs (at bacterial superhelical density) harboring the homopurine-homopyrimidine sequence, poly(dG)-poly(dC), were reacted with bromoacetaldehyde (BAA), a reagent that reacts with unpaired DNA bases. Not only did the poly(dG)-poly(dC) sequence react with BAA but, surprisingly, neighboring sequences located 3' to the contiguous G sequences also reacted. The altered conformation in the poly(dG)-poly(dC) sequence and in the neighboring sequence occurred in the same supercoiled plasmid DNA molecule. Furthermore, the occurrence of an "unpaired" conformation in the neighboring sequence is strictly due to a positional effect, since it is observed when the poly(dG)-poly(dC) segment is adjacent to a variety of neighboring sequences.

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Year:  1985        PMID： 4075394     DOI： 10.1016/0092-8674(85)90024-8

Source DB:  PubMed          Journal:  Cell        ISSN： 0092-8674            Impact factor:   41.582


  30 in total

1.  A single-stranded DNA binding protein from mouse tumor cells specifically recognizes the C-rich strand of the (AGG:CCT)n repeats that can alter DNA conformation.

Authors:  T Muraiso; S Nomoto; H Yamazaki; Y Mishima; R Kominami
Journal:  Nucleic Acids Res       Date:  1992-12-25       Impact factor: 16.971

2.  Reaction conditions affect the specificity of bromoacetaldehyde as a probe for DNA cruciforms and B-Z junctions.

Authors:  M J McLean; J E Larson; F Wohlrab; R D Wells
Journal:  Nucleic Acids Res       Date:  1987-09-11       Impact factor: 16.971

3.  Distinct patterns in the dinucleotide nearest neighbors to G/C and A/T oligomers in eukaryotic sequences.

Authors:  R Nussinov
Journal:  J Mol Evol       Date:  1991-09       Impact factor: 2.395

4.  DNA synthesis arrest sites at the right terminus of rat long interspersed repeated (LINE or L1Rn) DNA family members.

Authors:  E d'Ambrosio; A V Furano
Journal:  Nucleic Acids Res       Date:  1987-04-10       Impact factor: 16.971

5.  Nuclear activity from F9 embryonal carcinoma cells binding specifically to the enhancers of wild-type polyoma virus and PyEC mutant DNAs.

Authors:  F K Fujimura
Journal:  Nucleic Acids Res       Date:  1986-04-11       Impact factor: 16.971

6.  Identification of tissue specific nuclear proteins: DNA sequence and protein binding regions in the T cell receptor beta J-C intron.

Authors:  Y Hashimoto; A M Maxam; M I Greene
Journal:  Nucleic Acids Res       Date:  1990-05-25       Impact factor: 16.971

7.  Site-specific cleavage of supercoiled DNA by ascorbate/Cu(II).

Authors:  Y Wang; B Van Ness
Journal:  Nucleic Acids Res       Date:  1989-09-12       Impact factor: 16.971

8.  Formation of a parallel-stranded DNA homoduplex by d(GGA) repeat oligonucleotides.

Authors:  T Suda; Y Mishima; H Asakura; R Kominami
Journal:  Nucleic Acids Res       Date:  1995-09-25       Impact factor: 16.971

9.  Magnesium ion-dependent triple-helix structure formed by homopurine-homopyrimidine sequences in supercoiled plasmid DNA.

Authors:  Y Kohwi; T Kohwi-Shigematsu
Journal:  Proc Natl Acad Sci U S A       Date:  1988-06       Impact factor: 11.205

10.  Unusual conformational effect exerted by Z-DNA upon its neighboring sequences.

Authors:  T Kohwi-Shigematsu; T Manes; Y Kohwi
Journal:  Proc Natl Acad Sci U S A       Date:  1987-04       Impact factor: 11.205
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