Literature DB >> 4074745

A novel approach to the isolation of rabbit reticulocyte haem-controlled eIF-2 alpha protein kinase.

R J Jackson, T Hunt.   

Abstract

A new strategy for the purification of rabbit reticulocyte haem-controlled eIF-2 alpha kinase is described, based on the fact that this kinase can be self-phosphorylated in several sites. Incubation of partially purified kinase with ATP changes its behaviour on anion exchangers sufficiently to separate it from almost all contaminating proteins.

Entities:  

Mesh:

Substances:

Year:  1985        PMID: 4074745     DOI: 10.1016/0167-4781(85)90010-7

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN: 0006-3002


  2 in total

1.  eIF2 independently binds two distinct eIF2B subcomplexes that catalyze and regulate guanine-nucleotide exchange.

Authors:  G D Pavitt; K V Ramaiah; S R Kimball; A G Hinnebusch
Journal:  Genes Dev       Date:  1998-02-15       Impact factor: 11.361

2.  Activity of protein phosphatases against initiation factor-2 and elongation factor-2.

Authors:  N T Redpath; C G Proud
Journal:  Biochem J       Date:  1990-11-15       Impact factor: 3.857

  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.