| Literature DB >> 4074380 |
F Tchang, M Laroche-Raynal, C Vergnolle, C Demandre, D Douady, M Grosbois, F Guerbette, M Delseny, J C Kader.
Abstract
In order to study the biosynthesis of a plant phospholipid transfer protein (PLTP), poly (A)+RNAs have been prepared from maize seedlings and translated in vitro with a rabbit reticulocyte lysate. The newly synthesized proteins were then separated by fast protein liquid chromatography (FPLC) followed by SDS-PAGE or by high performance liquid chromatography (HPLC) coupled to a radioactivity detector monitor. It has been showed that a radioactive band comigrating with a 14C methylated pure PLTP was detected by SDS-PAGE. This result, confirmed by direct radioactive monitoring, indicates that PLTP is actively synthesized in vitro.Entities:
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Year: 1985 PMID： 4074380 DOI： 10.1016/0006-291x(85)91843-1
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575